Rapid Identification of Hemoplasma Species by Palindromic Nucleotide Substitutions at the GAAA Tetraloop Helix in the Specificity Domain of Ribonuclease P RNA
スポンサーリンク
概要
- 論文の詳細を見る
We have examined secondary structures of the ribonuclease P RNA sequences obtained from DNA databases, and identified a determinative prototype of the P12 helix peculiar to each species of hemoplasmas. This key structure will provide a rapid means for species identification of these uncultivable pathogens without making a phylogenetic tree based on alignments of nucleotide sequences. This procedure based on palindromic nucleotide substitutions at the stem portion of the P12 helix provide clear information such as the level of heterogeneity within a species, the relatedness between species, or facilitating the characterization and clustering of specific strains. In conclusion, the PNS analysis based on the evaluation of only the strategic and highly conserved genomic region in the specificity domain of RNase P RNA.
著者
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FUJIHARA Masatoshi
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate
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WATANABE Yusaku
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate
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Suzuki Jin
Department Of Food Safety Tokyo Metropolitan Institute Of Public Health
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Harasawa Ryo
Department Of Veterinary Medicine Faculty Of Agriculture Iwate University
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SASAOKA Fumina
Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University
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FUJIHARA Masatoshi
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University
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HARASAWA Ryo
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University
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WATANABE Yusaku
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020–8550, Japan
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WATANABE Yusaku
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University
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FUJIHARA Masatoshi
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020–8550, Japan
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SASAOKA Fumina
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020–8550, Japan
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SASAOKA Fumina
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University
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