Isolation of Maedi/visna Virus from a Sheep in Japan
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概要
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Maedi/visna (MV) is a lentiviral disease of sheep caused by the maedi/visna virus (MVV). Although MV is prevalent in many countries, it had not been reported in Japan. In 2011, however, three sheep in northern Japan were reported to be seropositive against the MVV antigen, indicating a persistent MVV infection. In the present study, we isolated MVV from one sheep to confirm MVV infection and conducted genomic classification of the virus. The co-culture of leukocytes from a seropositive sheep with fetal goat lung cells resulted in the formation of syncytial cells and the amplification of a long terminal repeat sequence of MVV by polymerase chain reaction. The isolate was confirmed as being MVV, rather than the caprine arthritis-encephalitis virus based on phylogenetic analysis of the gag gene sequence. Although the sheep was asymptomatic, nonpurulent meningitis and demyelination were found in the spinal cord. These were considered to be early lesions associated with pathogenic MVV infection. Therefore, the present study demonstrated that MVV is distributed in Japan.
著者
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Harasawa Ryo
Department Of Veterinary Medicine Faculty Of Agriculture Iwate University
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SENTSUI Hiroshi
Laboratory of Veterinary Epizootiology, Faculty of Veterinary Medicine, Nihon University
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Sasaki Jun
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
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GORYO Masanobu
Department of Applied Veterinary Science, United Graduate School of Veterinary Science, Gifu University
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GORYO Masanobu
Department of Veterinary Pathology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University
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OGUMA Keisuke
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University
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TANAKA Chiaki
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University
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KIMURA Atsushi
Veterinary Clinics of NOSAI Morioka
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HARASAWA Ryo
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University
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SENTSUI Hiroshi
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University
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