Efficient Propagation of Equine Viruses in a Newly Established Equine Cell Line, FHK-Tcl3.1 Cells
スポンサーリンク
概要
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Equine cells are required for isolation of viruses that infect the horse. However, only a few equine cell lines and cell cultures are available so far. Fetal horse kidney (FHK)-Tcl3.1 cell is a novel cell line established by introducing simian virus 40 (SV40) large T antigen. In the present study, the ability to propagate equine viruses was compared between FHK-Tcl3.1 cells and other equine cells. FHK-Tcl3.1 cells efficiently increased many viruses derived from or having pathogenicity to horses and produced high infective titers in culture fluids. These results indicate that FHK-Tcl3.1 cells would be useful for propagation and serological tests of viruses that affect Equidae.
著者
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Maeda Ken
Laboratory Of Fisheries Biology & Coral Reef Studies Faculty Of Science University Of The Ryukyu
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OGUMA Keisuke
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University
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OGUMA Keisuke
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252–0880, Japan
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SENTSUI Hiroshi
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University
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ISHIDA Maiko
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252–0880, Japan
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