Production and Characterization of Monoclonal Antibodies to Japanese Encephalitis Virus
スポンサーリンク
概要
- 論文の詳細を見る
In this study, eighteen monoclonal antibodies (MAbs) to recent Japanese encephalitis virus (JEV) genotype I were produced and characterized by plaque reduction neutralization test, western blot analysis, indirect immunofluorescence assay and enzyme-linked immunosorbent assay. All MAbs recognized only envelope (E) protein or conformational epitope of E and precursor membrane proteins. Two MAbs (7E5 and 3-3H8) possessed virus-neutralization activity, and their escape mutants possessed a change of glutamine to histidine at the position of 52 of E protein, suggesting that these neutralizing MAbs recognize the domain I–II hinge region of E protein. Five MAbs recognized all examined flaviviruses, and two were specific to JEV. These MAbs may be useful for differentiation and diagnosis of flaviviruses.
著者
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SHIMOJIMA Masayuki
Laboratory of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University
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SHIMODA Hiroshi
Laboratory of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University
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Maeda Ken
Laboratory Of Fisheries Biology & Coral Reef Studies Faculty Of Science University Of The Ryukyu
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Terada Yutaka
Laboratory Of Pasturing Disease National Institute Of Animal Hearth
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MAHMOUD Hassan
Laboratory of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University
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NOGUCHI Keita
Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
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TAKASAKI Tomohiko
Division 2, Department of Virology 1, National Institute of Infectious Diseases, 1–23–1 Toyama, Shinjuku, Tokyo 162–8640, Japan
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