Detection of lysozyme adsorption on titania using surface plasmon resonance on Au layer coated with thin titania film
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概要
- 論文の詳細を見る
Titanium metal, which is used as artificial bone, has a titania surface layer. In this study, titania was investigated using surface plasmon resonance (SPR) in terms of protein adsorption, which occurs during the initial process of cellular attachment by employing a multilayer device consisting of a titania layer on Au. The titania film was deposited on the Au layer in the multilayer device by atmospheric chemical vapor deposition. A flow cell was used to evaluate protein adsorption on the titania top layer of the multilayer device. Phosphor buffer solutions with and without the protein lysozyme (Lyz) were injected into the flow cell. From the attenuation of reflected light, the SPR angle was determined as the angle with minimum reflection intensity. The observed behavior of the SPR angle indicated that Lyz was adsorbed on the titania film surface, and the obtained SPR angles increased with the Lyz concentration on the titania surface. These results indicate that the titania/Au multilayer SPR device can easily be used to detect the adsorption of protein.
- 公益社団法人 日本セラミックス協会の論文
著者
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OHSHIO Shigeo
Department of Chemistry, Nagaoka University of Technology
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SAITOH Hidetoshi
Department of Chemistry, Nagaoka University of Technology
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Saitoh Hidetoshi
Department Of Bioresource Science Obihiro University Of Agriculture And Veterinary Medicine
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Nagao Tatsuya
Department Of Physics Faculty Of Science And Technology Keio University
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AKASAKA Hiroki
Department of Physical Chemistry, Hoshi University
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Ohshio Shigeo
Department Of Chemistry Nagaoka University Of Technology
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Akasaka H
Information Storage Products Department Nikon Corporation
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II Kiyoto
Department of Materials Science and Technology, Nagaoka University of Technology
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Akasaka Hiroki
Department Of Chemistry Nagaoka University Of Technology
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AKASAKA Hiroki
Department of Materials Science and Technology, Nagaoka University of Technology
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OHSHIO Shigeo
Department of Materials Science and Technology, Nagaoka University of Technology
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NAGAO Tatsuya
Department of Materials Science and Technology, Nagaoka University of Technology
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