Detection of Protein Adsorption on Silica Surface Using Surface Plasmon Resonance Sensor
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概要
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Rapid screening for exploring biocompatible ceramics is important. We demonstrate the detection of lysozyme (Lyz) on a silica surface using a custom-made surface plasmon resonance (SPR) sensor that can detect the interaction between ceramics and biomolecules. The results expressed as shifts of the resonance angle using the SPR sensor followed the expected theory when silica film was synthesized on the sensor chip. Moreover, the adsorption and desorption behaviors of Lyz on the hydrophilic or hydrophobic silica surface in phosphate buffer solution were investigated. Relatively large amounts of Lyz molecules aggregated on the hydrophobic silica surface.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2008-10-25
著者
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Saitoh Hidetoshi
Department Of Bioresource Science Obihiro University Of Agriculture And Veterinary Medicine
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Ohshio Shigeo
Department Of Chemistry Nagaoka University Of Technology
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Akasaka Hiroki
Department Of Chemistry Nagaoka University Of Technology
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Kishimoto Shinichi
Department Of Surgery Ii Osaka University Medical School
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Kishimoto Shinichi
Department of Materials Science and Technology, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan
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Saitoh Hidetoshi
Department of Materials Science and Technology, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan
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