1A1-C26 機能性ゲルマイクロツールを用いた局所閉空間環境センシング

概要

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A local pH sensing method in a microchip using a functional gel-microtool was newly developed. We used a salting-out gel-microtool impregnated with BromoThymol Blue (BTB), which is used as a pH indicator. The salting-out gel-microtool is made of ENT-3400, which is hydrophilic photo-crosslinkable resin. The primary constituent of this resin is polyethyleneglycol. A solution mixed with ENT-3400, BTB, and high concentrations of electrolytic solution are stirred, and a lot of microspheres impregnated with BTB are obtained. These microspheres are cured by UV illumination and adhere to the glass substrate, however, we can manipulate them one by one using the optical tweezers. Therefore, we can move and position it in the closed environment. Here we propose to use the salting-out gel-microtool with BTB as a carrier of pH indicator. We can measure pH value locally from the color of microtool using calibrated color information in YCrCb color space. We succeeded in measuring local pH value with the pH sensing gel-microtool by locating it at the desired point in the microchip.
一般社団法人日本機械学会の論文