Cloning, Expression, and Characterization of a Root-Form Phosphoenolpyruvate Carboxylase from Zea mays : Comparison with the C_4-Form Enzyme
スポンサーリンク
概要
- 論文の詳細を見る
A full-length cDNA for maize root-form phosphoenolpyruvate carboxylase (PEPC) was isolated. In the coding region, the root-form PEPC showed 76 and 77% identity with the C_4- and C_3-form PEPCs of maize, respectively, at the nucleotide level. At the amino acid level, the root-form was 81 and 85% identical to the C_4-and C_3-form PEPCs, respectively. The entire coding region was inserted into a pET32a expression vector so that it was expressed under the control of T7 promoter. The purified recombinant root-form PEPC had a V_<max> value of about 28 μmol min^<-1> (mg protein)^<-1> at pH 8.0. The K_m values of root-form PEPC for PEP and Mg^<2+> were one-tenth or less of those of C_4-form PEPC when assayed at either pH 7.3 or 8.0, while the value for HCO^-_3 was about one-half of that of C_4-form PEPC at pH 8.0. Glucose 6-phosphate and glycine had little effect on the root-form PEPC at pH 7.3; they caused two-fold activation of the C_4-form PEPC. The K_i(L-malate) values at pH 7.3 were 0.12 and 0.43 mM for the root-and C_4-form PEPCs, respectiely. Comparison of hydropathy profiles among the maize PEPC isoforms suggested that several streches of amino acid sequences may contribute in some way to their characteristic kinetic properties. The root-form PEPC was phosphorylated by both mammalian cAMP-dependent protein kinase and maize leaf protein kinase, and the phosphorylated enzyme was less sensitive to L-maleta.
- 日本植物生理学会の論文
著者
-
泉井 桂
近畿大学生物理工学部生物工学科
-
Izui Katsura
Laboratory Of Plant Physiology Graduate School Of Biostudies Kyoto University
-
Izui Katsura
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
-
Izui Katsura
Laboratory Of Plant Physiology Division Of Applied Biosciences Graduate School Of Agriculture Kyoto
-
HATA Shingo
Laboratory of Plant Physiology, Graduate School of Agriculture, Kyoto University
-
Kawamura Tatsuo
Department Of Electrical Engineering Shibaura Institute Of Technology
-
Masuda Takao
Div. Appl. Biosci. Grad. Sch. Agr. Kyoto Univ.
-
Kawamura T
Department Of Mathematics And Physics University Of Yamanashi
-
Masuda T
Div. Appl. Biosci. Grad. Sch. Agr. Kyoto Univ.
-
Dong Long-Ying
Laboratory of Plant Physiology, Graduate School of Agriculture, Kyoto University
-
Masuda Takao
Laboratory of Plant Physiology, Graduate School of Agriculture, Kyoto University
-
Kawamura Takao
Laboratory of Plant Physiology, Graduate School of Agriculture, Kyoto University
-
DONG Long-Ying
Div. Appl. Biosci., Grad. Sch. Agr., Kyoto Univ.
-
Hata S
Laboratory Of Plant Physiology Graduate School Of Biostudies Kyoto University
-
Hata Shingo
Graduate School Of Biostudies Kyoto University
-
Ishiyama Keiki
Graduate School Of Agricultural Science Tohoku University
-
Ishiyama Keiki
Riken Plant Science Center
-
Dong Long-ying
Div. Appl. Biosci. Grad. Sch. Agr. Kyoto Univ.
-
Kawamura Takao
Laboratory Of Plant Physiology Graduate School Of Agriculture Kyoto University:(present Address)sumi
-
Hata Shingo
Laboratory Of Crop Science Graduate School Of Bioagricultural Sciences Nagoya University
関連論文
- フェニックス・ロベレニーの空気浄化能力と室内設置効果
- X-Ray Standing Wave Analysis of GaAs/Si Interface
- X-Ray Standing Wave Analysis of Al/GaAs/Si(111)
- Differential Distribution of Proteins Expressed in Companion Cells in the Sieve Element-Companion Cell Complex of Rice Plants
- Breakdown Characteristics of SF_6 Gap Disturbed by a Metallic Protrusion under Oscillating Transient Overvoltages
- V-t Characteristics of SF6 Gap Disturbed by a Needle-Shaped Protrusion under Oscillating Transient Overvoltages
- Two-Dimensional In_Ga_As/GaAs Quantum Dot Superlattices Realized by Self-Organized Epitaxial Growth
- Magnetoluminescence Studies of Highly Packed InGaAs Self-organized Quantum Dots on GaAs(311)B
- The Procedure to Realize Two-Dimensional Quantum Dot Superlattices : From Incoherently Coupled to Coherently Coupled Quantum Dot Arrays
- 緩衝水溶液と接触した食品包装用プラスチック製品からの酵素活性化作用をもつ微量物質の溶出
- シックハウスガスを吸収除去する植物の開発
- 植物の炭酸固定酸素、ホスホエノールピルビン酸カルボキシラーゼ、を特異的にリン酸化するプロテインキナーゼとその調節
- 光合成のクロストーク (植物の代謝コミュニケーション--植物分子生理学の新展開) -- (クロストーク)
- Molecular Characterization of a Phosphoenolpyruvate Carboxylase from a Thermophilic Cyanobacterium, Synechococcus vulcanus with Unusual Allosteric Properties
- ホスホエールピルビン酸カルボキシラーゼの3次元構造 : そのアロステリック阻害機構
- CLONING AND CHARACTERIZATON OF PHOSPHOENOLPYRUVATE CARBOXYLASE (PEPC) GENE FROM THERMOPHILIC CYANOBACTERIUM Synechococcus vulcanus
- 1P06 ホスホエノールピルビン酸カルボキシラーゼのX線結晶構造解析
- ラン藻のゲノム構築と遺伝子操作 (ラン藻の分子構築とその機構)
- C4光合成の炭酸固定酵素とその遺伝子--構造・調節・進化
- cDNA Cloning of Squalene Synthase Genes from Mono- and Dicotyledonous Plants, and Expression of the Gene in Rice
- ON THE STRUCTURE AND EXPRESSION OF PLANT SQUALENE SYNTHASE
- Analyses of the transgenic rice plants expression antisense NADH-GOGAT RNA
- Cloning and Expression Analysis of a MAPKKK Gene and a Novel Nodulin Gene of Lotus japonicus
- Surfaces and Interfaces of High-T_c Superconductors for Contact and Junction Formation Studied with Synchrotron Radiation Photoemisison Spectroscopy : High Temperature Superconducting Thin-Films(Solid State Devices and Materials 1)
- Structure and Bonding at the CaF_2/GaAs(111) Interface : Surfaces, Interfaces and Films
- Cloning, Expression, and Characterization of a Root-Form Phosphoenolpyruvate Carboxylase from Zea mays : Comparison with the C_4-Form Enzyme
- A ROOT-FORM PEP CARBOXYLASE OF Zea mays : CHARACTERIZATION OF THE RECOMBINANT ENZYME
- REGULATORY PHOSPHORYLATION OF PEP CARBOXYLASE
- A Dithiothreitol-Sensitive Tetrameric Protease from Spinach Thylakoids Has Polyphenol Oxidase Activity
- CLONING OF A cDNA ENCODING ROOT-FORM PHOSPHO ENOLPYRUVATE CARBOXYLASE FROM MAIZE AND CONSTRUCTION OF AN EXPRESSION SYSTEM IN ESCHERICHIA COLI
- Proteolytic activity and polyphenol oxidase activity of dithiothreitol-sensitive protease from spinach
- Effects of Auxin and Anoxia on the Cell Wall Yield Threshold Determined by Negative Pressure Jumps in Segments of Cowpea Hypocotyl
- Temporary expression of the TAF10 gene and its requirement for normal development of Arabidopsis thaliana
- Knockdown of an Arbuscular Mycorrhiza-inducible Phosphate Transporter Gene of Lotus japonicus Suppresses Mutualistic Symbiosis
- Gln49 and Ser174 Residues Play Critical Roles in Determining the Catalytic Efficiencies of Plant Glutamine Synthetase
- Biochemical Background and Compartmentalized Functions of Cytosolic Glutamine Synthetase for Active Ammonium Assimilation in Rice Roots
- Light-Regulated Expression of the Gene for C_4-Form Phosphoenolpyruvate Carboxylase in Maize : Destabilization of mRNA by Okadaic Acid
- ANALYSES OF LIGHT-INDUCED EXPRESSION OF THE GENE FOR PHOSPHOENOLPYRUVATE CARBOXYLASE IN MAIZE
- REGULATORY PHOSPHORYLATION OFC_4-TYPE PEP CARBOXYLASE IN MAIZE
- Organization and Structure of Ferredoxin - Dependent Clutamate Synthase Gene and Intracellular Localization of the Enzyme Protein in Rice Plants
- Hole Transport in Glow-Discharge Produced a-Si:H:F Film
- Deposition Mechanism of a-Si:H Film in Disilane-Hydrogen Plasma
- Preparation and Some Properties of Highly Resistive a-Si:H:F Film
- Dynamic Mechanism of Fast Switching in Light Valve Using Liquid Crystal
- Localized Expression of Arbuscular Mycorrhiza-Inducible Ammonium Transporters in Soybean
- Thioredoxin-Mediated Reductive Activation of a Protein kinase for the Regulatory Phosphorylation of C4-from Phosphoenolpyruvete Carboxylase from Maize
- A SPECIFIC PROTEIN KINASE (PK) FOR MAIZE C4-FORM PEP CARBOXYLASE (PEPC) : PURIFICATION AND REGULATION BY THIOREDOXIN (Trx)
- Transient Impulse Breakdowns of SF_6 Gas in Inhomogeneous Electric Fields
- Cloning, Functional Expression, and Mutational Analysis of a cDNA for Lotus japonicus Mitochondrial Phosphate Transporter
- Characterization of a Saccharomyces cerevisiae Gene That Encodes a Mitochondrial Phosphate Transporter-Like Protein^1
- cDNA CLONING OF A MITOCHONDRIAL PHOSPHATE TRANSPORTER GENE FROM Lotus japonicus
- cDNA CLONING AND CHARACTARIZATION OF Lotus japonicus GENES FOR SQUALENE SYNTHASE
- PROMOTER ANALYSIS OF THE TWO SOYBEAN PEPC GENES : REGULATORY ELEMENTS AND NUCLEAR FACTORS REQUIRED FOR NODULE-ENHANCED EXPRESSION
- cDNA CLONING AND CHARACTERIZATION OF PHOSPHOENOLPYRUVATE CARBOXYLASE AND ITS PROTEIN KINASE OF Lotus japonicus ROOT NODULES
- Promoter analysis of a soybean nodule-enhanced PEPC gene
- Tissue and Cellular Localization of NADH-Dependent Glutamate Synthase Protein in Leaves of Spinach
- A Ca^-Dependent Protein Kinase that Endows Rice Plants with Cold- and Salt-Stress Tolerance Functions in Vascular Bundles
- LOCALIZATION OF STRESS-INDUCIBLE OsCDPK7 SIGNALING PATHWAY IN WILD-TYPE AND OsCDPK7-OVEREXPRESSING RICE PLANTS
- Cellular and subcellular localization of NADH-dependent or Fd-dependent glutamate synthase protein in the stele of rice roots
- Construction of a Plasmid for High Level Expression of Escherichia coli Phospho-enolpyruvate Carboxylase
- Effects of Site-directed Mutagenesis of Conserved Lys606 Residue on Catalytic and Regulatory Functions of Maize C_4-form Phosphoenolpyruvate Carboxylase
- High-level Expression of Maize C_4-type Phosphoenolpyruvate Carboxylase in Escherichia coli and Its Rapid Purification
- Site-directed mutagenesis of the two conserved lysine residues in phosphoenolpyruvate carboxylase from Zea mays
- Metal-Semiconductor Electroluminescent Diodes in ZnO Single Crystal
- An Anisotropy of Gain in N_2-Laser Pumped GaAs under Applied Electric Field
- Photoluminescence from Uudoped GaAs under Applied Electric Field : The Transition Mechanism at High Excitation Levels near 77 K
- Phosphoenolpyruvate Carboxylase of Eschericia coli--Hydrophobic Chromatography Using Specific Elution with Allosteric Inhibitor
- MOLECULAR CLONING AND FUNCTIONAL EXPRESSION OF cDNAs FOR MAIZE Ca^-DEPENDENT PROTEIN KINASEs(CDPKs)
- Abundant Expression in Vascular Tissue of Plant TAF10, an Orthologous Gene for TATA Box-binding Protein-associated Factor 10, in Flaveria trinervia and Abnormal Morphology of Arabidopsis thaliana Transformants on its Overexpression
- EXPRESSION ANALYSES OF PLANT MITOCHONDRIAL PHOSPHATE TRANSPORTERS
- Characterization of a Maize Ca^-Dependent Protein Kinase Phosphorylating Phosphoenolpyruvate Carboxylase
- FUNCTIONAL ANALYSIS OF OsCDPK7 IN STRESS RESPONSE USING Ds-INSERTION MUTANT OF RICE
- A SINGLE Ca^-DEPENDENT PROTEIN KINASE PROMOTES BOTH COLD-AND SALT/DROUGHT-TOLERANCE IN RICE PLANTS
- ALTERED COLD-STRESS TOLERANCE IN TRANSGENIC RICE PLANTS WITH VARIOUS LEVELS OF A STRESS-INDUCIBLE CALCIUM-DEPENDENT PROTEIN KINASE
- Changes in the tissue-localization of NADH-dependent glutamate synthase protein following the addition of NH_4^+ in the root-tips of rice seedlings
- Poto-Response Characteristics of Electrophtographic Plates in Nanosecond Periods
- CHARACTERIZATION OF A RICE COLD-STRESS-INDUCIBLE CALCIUM-DEPENDENT PROTEIN KINASE
- Growth Mechanism of Surface Dots Self-Assembled on InP (311)B Substrate
- Photoemission and RHEED Studies of Bonding Properties at the CaF_2/GaAs(001) Interface
- Dynamics of Periarbuscular Membranes Visualized with a Fluorescent Phosphate Transporter in Arbuscular Mycorrhizal Roots of Rice
- Signal Mediators for Phytoalexin Production in Defense Response of Oats Elicited by Victorin as a Specific Elicitor
- TOWARD CLARIFICATION OF THE RECOGNITION MECHANISM BETWEEN LOTUS JAPONICUS AND RHIZOBIA AT THE FINAL STAGE OF SYMBIOSIS
- ISOLATION AND CHARACTERIZATION OF A PEPC KINASE cDNA CLONE FROM A C4 PLANT, Flaveria trinervia
- ISOLATION AND CHARACTERIZATION OF A Flaveria trinervia cDNA FOR TBP-ASSOCIATED FACTOR, (FtTAF)_ 18
- Isolation and Characterization of cDNAs for Differentially Accumulated Transcripts between Mesophyll Cells and Bundle Sheath Strands of Maize Leaves
- CHARACTERIZATION OF NODULES FORMED BY Rhizobium etli ON Lotus japonicus ROOTS
- Ba_3Y_2Cu_2PtO_: Newly Obtained Phase During Single Crystal Growth of Ba_2YCu_3O_ from Platinum Crucide
- Histochemical studies on the expression of two glutamate synthase proteins and mRNAs in rice roots
- NOVEL PROTEINS SPECIFIC TO C4 PLANTS
- cDNA CLONING OF GENES EXPRESSED AT EARLY STAGE OF FLOWER INDUCTION IN APICAL BUDS OF Pharbitis nil
- MOLECULAR CLONING OF cDNAs FOR PLANT MITOCHONDRIAL PHOSPHATE TRANSPORTER
- EXPRESSION OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN NITROGEN-FIXING NODULES OF SOYBEAN ROOTS
- Identification and Expression Analysis of Arbuscular Mycorrhiza-Inducible Phosphate Transporter Genes of Soybean
- Expression of Plant Genes for Arbuscular Mycorrhiza-Inducible Phosphate Transporters and Fungal Vesicle Formation in Sorghum, Barley, and Wheat Roots