Organization and Structure of Ferredoxin - Dependent Clutamate Synthase Gene and Intracellular Localization of the Enzyme Protein in Rice Plants

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概要

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• A 5822bp long cDNA clone encoding the full length ferredoxin-dependent glutamate synthase (Fd-GOGAT; EC 1.4.7.1) protein was isolated from roots of rice (Oryza sativa L. cv. Sasanishiki). Its sequence was identical to those of partial cDNAs for Fd-GOGAT from green leaves and shoots of rice. The predicted open reading frame (4848bp) encodes a 1616 amino acid protein with a molecular mass of 175034 Da that includes a 96-amino acid presequence. The combined nucleotide sequence of genomic clones for Fd-GOGAT isolated from rice was 20899 bp long and contained an entire structural gene, a 5672bp 5’-upstream region from the first methionine and a 779bp 3’-downstream region from the stop codon. The predicted transcribed region (15.4kb) consisted of 33 exons separated by 32 introns. The evolution among diverse GOGAT proteins, expression of Fd-GOGAT gene and intracellular localization of this protein in rice roots are also described.

• 日本植物細胞分子生物学会の論文
• 2003-03-03

著者

• YAMAYA Tomoyuki

  Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University

• 泉井 桂

  近畿大学生物理工学部生物工学科

• 泉井 桂

  京都大院・農・植物生理

• Sakai T

  Tokyo Inst. Technol. Kanagawa Jpn

• Goto Satoshi

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Izui Katsura

  Laboratory Of Plant Physiology Graduate School Of Biostudies Kyoto University

• Izui Katsura

  Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University

• Izui Katsura

  Laboratory Of Plant Physiology Division Of Applied Biosciences Graduate School Of Agriculture Kyoto

• Sakai Tomohiro

  Department Of Innovative And Engineered Materials Interdisciplinary Graduate School Of Science And E

• Yamaya Tomoyuki

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Yamaya T

  Tohoku Univ. Sendai Jpn

• Yamaya T

  Institute For Agricultural And Biological Sciences. Okayama University

• Yamaya Tomoyuki

  Laboratory Of Plant Cell Engineering Department Of Agricultural Chemistry Faculty Of Agriculture Toh

• Yamaya Tomoyuki

  Graduate School Of Agricultural Science Tohoku University

• Yamaya Tomoyuki

  Dept. Applied Plant Sci. Graduate School Of Agric. Sci. Tohoku Univ.

• Yamaya Tomoyuki

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Yamaya Tomoyuki

  Department Of Applies Plant Science Graduate School Of Agricultural Science Tohoku University

• Hayakawa Toshihiko

  Laboratory of Plant Nutrition and Laboratory of Cell Biochemistry, Department of Applied Biological

• Goto Satoshi

  Laboratory For Cerebrovascular Disorders And Neuroscience Department Of Neurosurgery Kumamoto Univer

• Nakajima Hiroyuki

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Sakai Tatsuya

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Hayakawa Toshihiko

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Hayakawa Toshihiko

  Graduate School Of Agric. Sci. Tohoku Univ.

• Ishiyama Keiki

  Graduate School Of Agricultural Science Tohoku University

• Ishiyama Keiki

  Riken Plant Science Center

• Hirose Naoya

  Metabolic Function Group Plant Science Center Riken Yokohama Institute

• SAKAI Takahiro

  Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science

• ISHIYAMA Keiki

  Metabolic Function Group, Plant Science Center, RIKEN Yokohama institute

• TAKEZAWA Masae

  Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science

• NAITO Kazutaka

  Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science

• HINO-NAKAYAMA Mutsumi

  Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science

• AKAGAWA Takumi

  Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science

• Takezawa Masae

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Naito Kazutaka

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Akagawa Takumi

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Sakai T

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Hino Mutsumi

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Sakai Toshikatsu

  School Of Science And Engineering Waseda University:crest Japan Science And Technology Corporation (

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