Tissue and Cellular Localization of NADH-Dependent Glutamate Synthase Protein in Leaves of Spinach

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概要

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• Tissue and cellular localization of NADH-dependent glutamate synthase (NADH-GOGAT, EC1.4. 1.14) in young leaves of dicotyledonous spinach (Spinacia oleacea) was investigated using the immunocytological method with an affinity-purified anti-NADH-GOGAT immunoglobulin G. Immunoblotting analysis showed this antibody specifically cross-reacted with NADH-GOGAT protein in crude soluble proteins from young leaf blades of spinach. When transverse sections (10μm in thickness) prepared from the paraffin-embedded young leaf blades of spinach were stained with the anti-NADH-GOGAT antibody, strong signals for NADH-GOGAT protein were detected in companion cells of large vascular bundles. Weak signals for the NADH-GOGAT protein were also detected in vascular parenchyma cells and mesophyll cells of young leaves. Ferredoxin (Fd)-GOGAT (EC 1.4.7.1) protein was mainly located in mesophyll cells and signals for the protein were also detected in companion cells and xylemparenchyma cells of large vascular bundles. By the way, in young leaf blades of monocotyledonous rice, NADH-GOGAT protein specifically located in vascular cells and Fd-GOGAT protein was abundant in mesophyll cells (Hayakawa et al., 1994). The differences of functions in young leaves for NADH-GOGAT proteins between monocotyledonous rice and dicotyledonous spinach are discussed.

• 東北大学の論文

著者

• YAMAYA Tomoyuki

  Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University

• 泉井 桂

  近畿大学生物理工学部生物工学科

• 泉井 桂

  京都大院・農・植物生理

• Izui Katsura

  Laboratory Of Plant Physiology Graduate School Of Biostudies Kyoto University

• Izui Katsura

  Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University

• Izui Katsura

  Laboratory Of Plant Physiology Division Of Applied Biosciences Graduate School Of Agriculture Kyoto

• YAMAYA Tomoyuki

  Graduate School of Agricultural Science, Tohoku University

• Yamaya Tomoyuki

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Yamaya T

  Tohoku Univ. Sendai Jpn

• Yamaya T

  Institute For Agricultural And Biological Sciences. Okayama University

• Yamaya Tomoyuki

  Graduate School Of Agric. Sci. Tohoku Univ.

• Yamaya Tomoyuki

  Graduate School Of Agricultural Science Tohoku University

• Yamaya Tomoyuki

  Dept. Applied Plant Sci. Graduate School Of Agric. Sci. Tohoku Univ.

• HAYAKAWA Toshihiko

  Graduate School of Agricultural Science, Tohoku University

• Yamaya Tomoyuki

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Yamaya Tomoyuki

  Department Of Applies Plant Science Graduate School Of Agricultural Science Tohoku University

• Hayakawa Toshihiko

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Hayakawa Toshihiko

  Graduate School Of Agric. Sci. Tohoku Univ.

• Ishiyama Keiki

  Graduate School Of Agricultural Science Tohoku University

• Ishiyama Keiki

  Riken Plant Science Center

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