Construction of a Plasmid for High Level Expression of Escherichia coli Phospho-enolpyruvate Carboxylase
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概要
- 論文の詳細を見る
The minimum size DNA fragment (3011 bp) containing the entire phosphoenolpyruvate carboxylase [EC 4.1.1.31] gene of E. coli was cloned into a modified plasmid vector of high copy-number. The gene expression was directed by its own promoter and the content of the enzyme reached about 30% of total soluble protein of the transformed cells.
- 社団法人日本農芸化学会の論文
- 1995-04-23
著者
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泉井 桂
近畿大学生物理工学部生物工学科
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Izui Katsura
Department Of Agricultural Biology Faculty Of Agriculture Kyoto University
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Fujita Nobuyuki
Department Of Chemistry Faculty Of Science Kyoto University
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Fujita Nobuyuki
Department Of Cell Differentiation Sakaguchi Laboratory Of Developmental Biology Keio University Sch
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TERADA Kazutoyo
Department of Molecular Genetics, Kumamoto University School of Medicine
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Ishiyama Keiki
Graduate School Of Agricultural Science Tohoku University
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Ishiyama Keiki
Riken Plant Science Center
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Terada Kazutoyo
Department Of Chemistry Faculty Of Science Kyoto University
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Katsuki Hirohiko
Department of Chemistry, Faculty of Science, Kyoto University
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Katsuki Hirohiko
Department Of Chemistry Faculty Of Science Kyoto University
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