Preparation and characterization of monoclonal antibodies against phosphoenolpyruvate carboxylase of Escherichia coli.
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概要
- 論文の詳細を見る
Twelve hybridoma clones which secrete monoclonal antibodies (mAb) against purified phosphoenolpyruvate carboxylase [EC 4.1.1.31] from Escherichia coli K-12 were obtained. These 12 mAb were prepared from the ascites fluids of mice. Six among the 12 mAb formed precipitin lines with the enzyme on immunodiffusion. Four mAb inhibited the activity of the enzyme and 2 mAb enhanced it. Four mAb altered the sensitivity of the enzyme to allosteric effectors. Competitive enzyme-binding experiments among the 12 different mAb were also performed. The results showed that the 12 mAb can be classified into at least 8 groups.
- 社団法人 日本生化学会の論文
著者
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Izui Katsura
Department Of Agricultural Biology Faculty Of Agriculture Kyoto University
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Hanaoka Masao
Department Of Pathology Institute For Virus Research Kyoto University
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Hirai Keisuke
Department Of Pathology Wakayama Medical School
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Ishijima Sumio
Department Of Biochemistry Chiba University
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Taguchi Masaaki
Department of Applied Chemistry, Faculty of Engineering, Tohoku University
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TAGUCHI Masaaki
Department of Pathology, Institute for Virus Research, Kyoto University
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NAMBA Yuziro
Department of Pathology, Institute for Virus Research, Kyoto University
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KATSUKI Hirohiko
College of Liberal Arts, Kinki University
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HIRAI Keisuke
Department of Chemistry, Faculty of Science, Kyoto University
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