Purification and Properties of Fatty Acid Hydroperoxide Lyase from Green Bell Pepper Fruits : PROTEINS, ENZYMES AND METABOLISM : NATURAL PRODUCTS
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概要
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Fatty acid hydroperoxide lyase (HPO lyase) was purified to apparently homogeneity state from immature fruits of green bell pepper (Capsicum annuum L.) by differential centrifugation, ion-exchange chromatography, hydroxylapatite chromatography and gel filtration. The enzymatic activity was separated into two fractions (HPO lyases I and II) during the chromatography on hydroxylapatite. Both the isoforms were deduced to be trimers of 55-kDa subunits and have similar enzymatic properties. Peptide maps revealed only slight differences between them. Furthermore, immunoblot analysis showed that an antibody raised against HPO lyase I reacted with HPO. lyase 11 as strongly as with the original antigen. These results indicate that there is only limited heterogeneity in terms of amino acid sequence and/or post-translational modification. The activities of both HPO lyases were considerably inhibited by lipophilic antioxidants, such as nordihydroguaiaretic acid and α-tocopherol. The activities with 13-hydroperoxy-(9Z, 11E, 15Z)-octadecatrienoic acid as substrate were about 12 times higher than those with 13-hydroperoxy-(9Z, 11E)-octadecadienoic acid. By contrast, no reactivity was detectable against the geometrical isomer, 13-hydroperoxy-(9E, 11L)-octadecadienoic acid or against the positional isomer, 9-hydroperoxy-(10E, 12Z)-octadecadienoic acid. Tissue-print immunoblot analyses using antiserum against HPO lyase indicated that HPO lyase was most abundant in the outer parenchymal cells of the pericarp.
- 日本植物生理学会の論文
著者
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MATSUI Kenji
Department of Biological Chemistry, Faculty of Agriculture and Applied Molecular Bioscience, Graduat
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KAJIWARA Tadahiko
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Matsui K
Yamaguchi Univ. Yamaguchi Jpn
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Matsui Kenji
Dep. Of Biological Chemistry Fac. Of Agriculture And Dep. Of Applied Molecular Medicine Graduate Sch
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Matsui Kenji
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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SHIBATA Yasushi
Department of Applied Biological Science, Faculty of Science and Technology
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HATANAKA Akikazu
Department of Agricultural Chemistry Faculty of Agriculture, Yamaguchi University
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Shibata Yasushi
Department Of Biological Chemcistry Faculty Of Agriculture Yamaguchi University
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Shibata Yasushi
Department Of Agricultural Chemistry Faculty Of Agriculture Yamaguchi University
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Hatanaka Akikazu
Department Of Life Science Graduate School Of Integrated Arts And Science University Of East Asia
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Hatanaka Akikazu
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Yamaguchi
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Kajiwara Tadahiko
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Yamaguchi
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Matsui Kenji
Department Of Biological Chemistry Faculty Of Agriculture And Department Of Applied Molecular Medici
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Matsui Kenji
Department Of Biological Chemistry Faculty Of Agriculture And Applied Molecular Bioscience Graduate
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