Effects of inhibitors on the enzyme system producing C_6-aldehydes frown C_<18>-unsaturated fatty acids in chloroplasts of Japanese silver (Farfugium japonicum) leaves
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概要
- 論文の詳細を見る
When chloroplasts isolated from Farfugium japonicum (Japanese silver) leaves were used as an enzyme source, the activity of the enzyme system producing C_6-aldehydes (cis-3-hexenal and n-hexanal) from C_<18>-unsaturated fatty acids (linolenic and linoleic acids) decreased upon treatment with LAHase from potato. This enzyme system could not be separated from chlorophylls and lipids by detergent treatment and was not affected by light illumination, CCCP or DCMU. The activity of the enzyme system was inhibited by MB and NTB used as a redox reagent, SKF 525-A as an oxidase inhibitor and DABCO as a quencher of singlet oxygen, but not by DCIP, PMS and SOD. These data suggest that; i) interaction of the enzyme system with lipids is required for maximal enzyme activity, ii) this enzyme system may involve electron mediator(s), and iii) singlet oxygen takes part in the enzyme reaction.
- 日本植物生理学会の論文
著者
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KAJIWARA Tadahiko
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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HATANAKA Akikazu
Department of Agricultural Chemistry Faculty of Agriculture, Yamaguchi University
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SEKIYA Jiro
Department of Agricultural Chemistry Faculty of Agriculture, Yamaguchi University
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Sekiya Jiro
Div. Appli. Life. Sci. Grad. Sch. Agr. Kyoto Univ.
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Sekiya J
Department Of Agricultural Sciences Faculty Of Agriculture Okayama University:(present Address) Depa
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Sekiya Jiro
Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.
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Hatanaka Akikazu
Department Of Life Science Graduate School Of Integrated Arts And Science University Of East Asia
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Hatanaka Akikazu
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Yamaguchi
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Sekiya Jiro
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Kajiwara Tadahiko
Department Of Agricultural Chemistry Faculty Of Agriculture Yamaguchi University
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Kajiwara Tadahiko
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Yamaguchi
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