Cloning and molecular analysis of radish (Raphanus sativus L.) cDNAs encoding heterodimeric γ-glutamyltransferases
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概要
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γ-Glutamyltransferase (GGT) catalyzes the hydrolysis and transpeptidation of the γ-glutamyl moiety of γ-glutamyl peptides. Based on the N-terminal amino acid sequences of purified radish heterodimeric GGTs, we cloned and characterized three radish full-length cDNAs (RsGGT1, RsGGT2 and RsGGT3) encoding putative heterodimeric GGT isoforms. RsGGT proteins contained conserved amino acid residues that are required for the catalytic activity and the post-translational processing of GGT proteins in E. coli and mammals. Expression analysis indicated that RsGGT showed different organ expression patterns. The overexpression of RsGGT1 and RsGGT2 cDNAs, but not that of RsGGT3 cDNA, resulted in an increase of NaCl-extractable bound GGT activity in transgenic tobacco plants. These results suggest that RsGGT1 and RsGGT2 cDNAs encode heterodimeric bound GGT isoforms.
- 日本植物細胞分子生物学会の論文
- 2006-09-01
著者
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Sekiya Jiro
Div. Appli. Life. Sci. Grad. Sch. Agr. Kyoto Univ.
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Sekiya J
Department Of Agricultural Sciences Faculty Of Agriculture Okayama University:(present Address) Depa
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Sekiya Jiro
Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.
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Nakano Yoshihiro
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sekiya Jiro
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sekiya Jiro
Division Of Applied Life Sciences Graduate School Of Agricultural Sciences Kyoto University
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PRIETO Rafael
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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HARA Masakazu
Fatuity of Agriculture, Shizuoka University
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Hara Masakazu
Fatuity Of Agriculture Shizuoka University
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