Purification and Properties of Soluble and Bound γ-Glutamyltransferases from Radish Cotyledon
スポンサーリンク
概要
- 論文の詳細を見る
Soluble and cell wall bound γ-glutamyltransferases (GGTs) were purified from radish (Raphanus sativus L.) cotyledons. Soluble GGTs (GGT I and II) had the same Mr of 63,000, and were composed of a heavy subunit (Mr, 42,000) and a light one (Mr, 21,000). The properties of GGT I and II were similar. Bound GGTs (GGT A and B) were purified to homogeneity from the pellet after the extraction of soluble GGTs. GGT A and B were monomeric proteins with an Mr of 61,000. The properties of GGT A and B were similar. Thus, bound GGTs were distinguished from soluble GGTs. The optimal pHs of soluble and bound GGTs were about 7.5. Both soluble and bound GGTs utilized glutathione, γ-L-glutamyl-p-nitroanilide, oxidized glutathione and the conjugate of glutathione with monobromobimane as substrates, and were inhibited by acivicin, but soluble GGTs were also distinguished from bound GGTs with regard to these properties.
- 社団法人 日本農芸化学会の論文
- 2006-02-23
著者
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Koizumi Yukio
Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.
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Koizumi Yukio
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sekiya Jiro
Div. Appli. Life. Sci. Grad. Sch. Agr. Kyoto Univ.
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Sekiya J
Department Of Agricultural Sciences Faculty Of Agriculture Okayama University:(present Address) Depa
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Sekiya J
Okayama Univ. Okayama Jpn
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Sekiya Jiro
Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.
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Nakano Yoshihiro
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sekiya Jiro
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sekiya Jiro
Division Of Applied Life Sciences Graduate School Of Agricultural Sciences Kyoto University
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Okawa Satoshi
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Okawa S
Department Of Computer Software The University Of Aizu
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YAMAUCHI Takayoshi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Yamauchi Takayoshi
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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