Non-radioactive Adenosine 5'-Phosphosulfate Sulfotransferase Assay by Coupling with Sulfite Reductase and O-Aietylserine(thiol)lyase
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概要
- 論文の詳細を見る
Adenosine 5'-phosphosulfate (APS) sulfotransferase is thought to be an enzyme that transfers the sulfo-group of APS to a carrier compound with a thiol group in the assimilatory sulfate reduction pathway of higher plants. We developed a rapid, non-radioactive assay for APS sulfotransferase. Sulfite released by APS sulfotfansferase reaction in the presence of excess dithiothreitol was further converted to cysteine by coupling with yeast sulfite reductase and cabbage O-acetylserine(thiol)lyase. The cysteine thus formed was measured colorimetrically. By this method, 5 to 300 nmol of sulfite could be assessed. When the method was applied to APS sulfotransferase, the enzyme activity was APS-dependent with the partially purified enzyme. We could also detect APS sulfotransferase activity in some higher plants by this method.
- 社団法人日本農芸化学会の論文
- 1997-04-23
著者
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Ara Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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SEKIYA Jiro
Department of Agricultural Chemistry Faculty of Agriculture, Yamaguchi University
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Sekiya Jiro
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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