Molecular Cloning and Expression of Arabidopsis Fatty Acid Hydroperoxide Lyase
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概要
- 論文の詳細を見る
Fatty acid hydroperoxide lyase(HPOL), an enzyme of the octadecanoid pathway that forms carbon-6 aldehydes such as n-hexanal or(Z)-3-hexenal, was cloned from Arabidopsis thaliana as a full-length cDNA. The HPOL activity obtained by expressing the cDNA in Escherichia coli formed n-hexanal from linoleic acid 13-hydroperoxide, whereas linoleic acid 9-hydroperoxide was not a substrate for the enzyme. The HPOL mRNA is expressed at low level in leaves; however, its accumulation can be found in the inflorescence. Wounding or methyl jasmonate treatments increase the mRNA level in leaves. These results indicate that the HPOL gene is up-regulated in leaves in response to wounding and that the enzyme may be an active component of the octadecanoid defense response.
- 日本植物生理学会の論文
著者
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MATSUI Kenji
Department of Biological Chemistry, Faculty of Agriculture and Applied Molecular Bioscience, Graduat
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KAJIWARA Tadahiko
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Matsui K
Yamaguchi Univ. Yamaguchi Jpn
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Matsui Kenji
Dep. Of Biological Chemistry Fac. Of Agriculture And Dep. Of Applied Molecular Medicine Graduate Sch
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Matsui Kenji
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Wilkinson Jack
Calgene Llc
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Hiatt Bill
Calgene Llc
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KNAUF Vic
Calgene, LLC
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Knauf Vic
Calgene Llc
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Kajiwara Tadahiko
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Yamaguchi
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Matsui Kenji
Department Of Biological Chemistry Faculty Of Agriculture And Department Of Applied Molecular Medici
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Matsui Kenji
Department Of Biological Chemistry Faculty Of Agriculture And Applied Molecular Bioscience Graduate
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