フェノキサゾン化合物の生体物理化学的研究(第8報) : 水晶体タンパク質の光散乱曲線におよぼすフェノキサゾン化合物の影響

概要

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In order to study the interaction of soluble protein of bovine lens and phenoxazone compounds, examinations were made on α- and β-crystallin solution added with phenoxazone compounds, using the precipitation-titration method with light-scattering photometer. Examination of the effect of concentration of protein solution on the light-scattering curve showed that 0.2% of the phenoxazone compound added to α-crystallin solution and 0.001% added to β-crystallin solution were the optimal concentration, and that completely desalted protein solution must be used. Further examinations were made on the addition of 14 kinds of compounds, including phenoxazone compounds and quinone derivatives, and it was found that the addition of pyrido-phenoxazone compound resulted in the decrease of first maximum at pH 5.5 of the light-scattering curve in the direction of 45° and 90° in α-crystallin. The degree of this decrease varied with the concentration of the additive but no change was seen in the scattered light in the direction of 135° and transmitted light in the direction of 0°. In the case of β-crystallin, entirely no effect was found with phenoxazone and other compounds.
公益社団法人日本薬学会の論文
1966-03-25