Mitomycinと血清Albuminの結合(第3報)Bovine Serum Albuminに対するMitomycinの結合量の分配平衡法による測定

概要

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Following the previous work on the interaction between mitomycin derivatives and bovine serum albumin (BSA) at pH 7.0 and 8.0,by the membrane equiliblium method, which showed pH dependence of this interaction and association constants at pH 7.0 1.6-3.3 times larger than those at pH 8.0,effect of pH on the binding of mitomycins and BSA was examined in the pH region of 6.0 to 10.0 by the partition equilibrium method based on the distribution of mitomycins between heptanol and aqueous buffer solution. It was found that in the pH region of 6.0 to 8.0,the number of binding sites of mitomycins on a BSA molecule was about 9,as was revealed by membrane equilibrium method, and analysis of the data by the Scatchard method revealed that there existed, at least, two classes of binding sites. In the pH region of 9.0 to 10.0 the number of binding sites decreased to 4-6 with homogeneous binding sites. The apparent association constants k_1 of the first binding class decreased with the increase of pH above 7.0,and linear relationship was observed between log k_1 and pH. The difference in these results obtained by membrane equilibrium method and by partition equilibrium method was assumed to be due to the denaturation of BSA by heptanol.
公益社団法人日本薬学会の論文
1974-11-25