薬用人参のサポニンの定量法
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概要
- 論文の詳細を見る
A new method for quantitative analysis of ginseng saponins, ginsenoside-Ro, -Rb_1,-Rb_2,-Rc, -Rd, -Re, -Rf, -Rg_1,and -Rg_2,was developed by the combination of thin-layer chromatography (TLC) and spectrometry. The 90% methanol extract of ginseng and pure standard ginsenosides were spotted on the same two TLC plates ; one plate was developed with chloroform- methanol-water (65 : 35 : 10,lower phase) and the other with chloroform-butanol-methanol-water (20 : 40 : 15 : 20,lower phase). After drying, both plates were sprayed with 10% sulfuric acid solution followed by heating, and each ginsenoside spot was measured by TLC densitometer using dual-wave length TLC scanner. Reasonable recovery and standard deviation were found in this procedure and the contents of each ginsenoside in ginseng and its congeners, American ginseng, San-chi ginseng, were determined by applying the described method.
- 公益社団法人日本薬学会の論文
- 1978-08-25
著者
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庄司 順三
昭和大学薬学部
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柴田 承二
Meiji College of Pharmacy
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真田 修一
School Of Pharmaceutical Sciences Showa University Hatanodai
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柴田 承二
明治薬科大学
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- サポニン
- 生薬学の過去, 現在, 将来(生薬学の現状と将来)
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