Purification of a Growth-Suppressing Factor for Bovine Artery Endothelial Cells from Mouse Lymphoma P388D1 Cells
スポンサーリンク
概要
- 論文の詳細を見る
A growth-supperssing factor for bovine artery endothelial cells (BAEGSF) was purified from the conditioned medium of a mouse lymphoma P388D1 cell culture in the presence of 100 μg/ml carboxymethylated curdlan. The purification steps included, in order, ammonium sulfate fractionation and eight stafes of column chromatography on Macro-Prep Ceramic Hydroxyapatite, Q-Sepharose, Sephacryl S-300 HR, Matrex PBA-30,CHT II, Resource-Q, anti-bovine serum albumin (BSA) agarose, and Superdex 200HR columns. The purified BAEGSF showed two bands with silver staining on a sodium dodecyl sulfate polyacrylamide gel under reducing conditions (SDS-PAGE) and their molecular weights were estimated as approximately 55 and 63 kDa, while the molecular weight of the purified BAEGSF was estimated as about 65 kDa by gel filtration using Superdex 200HR. This result shows that BAEGSF obtained from Superdex 200HR chromatography is a partialy purified preparation and suggests that one of the two bands on SDS-PAGE corresponds to BAEGSF. BAEGSF was shown not to have a lethal effect on endothelial cells, but had an inhibitory action on the proliferation of these cells. Furthermore, the growth-suppressing activity of BAEGSF for bovine artery endothelial cells (BAE) was not inhibited by anti-transforming growth factor-β (TGF-β), anti-tumor necrosis factor-α (TNF-α), and anti-interleukin-1 (IL-1) antibodies. These results suggest that BAEGSF is different from TGF-β, TNF-α, and IL-1 wich have been reported to inhibit BAE growth.
- 公益社団法人日本薬学会の論文
- 1999-01-15
著者
-
HIRANO Kazuyuki
Gifu Pharmaceutical University
-
HIRANO Kazuyuki
Department ofPharmaceutics, Glfu Pharmaceutical University
-
MATSUNAGA Toshiyuki
Department of Biochemistry, Research Center for Genomic Medicine, Saitama Medical School
-
Hirano K
Lab. Of Pharmaceutics Gifu Pharmaceutical Univ.
-
Hirano K
Gifu Pharmaceutical University
-
USUI Shigeyuki
Department of Pharmaceutics, Gifu Pharmaceutical University
-
KIHO Tadashi
Gifu Prefectural Institute of Health and Environmental Sciences
-
KIHO Tadashi
Department of Pharmaceutics, Gifu Pharmaceutical University
-
UKAI Shigeo
Department of Pharmaceutics, Gifu Pharmaceutical University
-
Kiho T
Gifu Prefectural Institute Of Health And Environmental Sciences
-
Kiho Tadashi
Gifu Pharmaceutical University
-
Usui Shigeyuki
Laboratory Of Pharmaceutics Gifu Pharmaceutical University
-
Hirano Kazuyuki
Department Of Material Chemistry Graduate School Of Engineering Kyoto University
-
Ukai S
Gifu Pharmaceutical Univ. Gifu Jpn
-
Kiho T
Gifu Pharmaceutical Univ. Gifu Jpn
-
Horie Kazutoshi
Developmental Research Laboratories Shionogi & Co. Ltd.
-
Matsunaga Toshiyuki
Department Of Biochemistry Research Center For Genomic Medicine Saitama Medical School
-
HIRANO Kazuyuki
Department of Clinical Chemistry School of Health Sciences, Fujita Health University
関連論文
- Increased Expression of Sucrase and Intestinal-type Alkaline Phosphatase in Human Gastric Carcinomas with Progression
- Gastric Tumorigenicity of 1,2-Dimethylhydrazine on the Background of Gastric Intestinal Metaplasia Induced by X-Irradiation in CD (SD) Rats
- Apparent Increase of Insulin Peak Area in HPLC Analysis of a Preparation Consisting of a Mixture of Insulin and Total Parenteral Nutrition
- P-254 SYNTHESIS AND ANTI-PROLIFERATIVE ACTIVITY OF N-(ω-DIPHENYLALKYL)-4-PHENYLPIPERIDINES DESIGNED FROM OPIOID DERIVATIVES
- Activation of Caspase-3, Proteolytic Cleavage of DFF and No Oligonucleosomal DNA Fragmentation in Apoptotic Molt-4 Cells
- Zinc Induces Mixed Types of Cell Death, Necrosis, and Apoptosis, in Molt-4 Cells.
- Zinc Induces Mixed Types of Cell Death, Necrosis, and Apoptosis, in Molt-4 Cells^1
- Decreased Thymosinβ_4 in Apoptosis Induced by a Variety of Antitumor Drugs.
- CLOCK/BMAL1 is Involved in Lipid Metabolism via Transactivation of the Peroxisome Proliferator-activated Receptor (PPAR) Response Element
- Apoptosis of Endothelial Cells may be Mediated by Genes of Peroxisome Proliferator-activated Receptor γ1 (PPAR γ1) and PPARα Genes
- Detection of oxidized high-density lipoprotein
- Modulation of Reactive Oxygen Species in Endothelial Cells by Peroxynitrite Treated Lipoproteins.
- Glycated High-Density Lipoprotein Induces Apoptosis of Endothelial Cells via Mitochondrial Dysfunction.
- Modulation of Reactive Oxygen Species in Endothelial Cells by Peroxynitrite-Treated Lipoproteins
- Reactive Oxygen Species as a Risk Factor in Verotoxin-1-Exposed Rats.
- Aminopeptidase N Regulated by Zinc in Human Prostate Participates in Tumor Cell Invasion.
- Decreases of Metallothionein and Aminopeptidase N in Rental Cancer Tissues.
- Decreases of Metallothionein and Aminopeptidase N in Renal Cancer Tissues
- Hydrolytic Profile for Ester- or Amide-linkage by Carboxylesterases pI 5.3 and 4.5 from Human Liver
- Variable Glyceryl Dinitrate Formation as a Function of Glutathione S-Transferase
- Inhibition of Purified Human Sucrase and Isomaltase by Ethanolamine Derivatives
- Preventive Effect of Co-administration of Water Containing Magnesium Ion on Indomethacin Induced Lesions of Gastric Mucosa in Adjuvant-Induced Arthritis Rat(Biopharmacy)
- POLYMORPHISM OF EXTRACELLULAR SUPEROXIDE DISMUTASE (EC-SOD) GENE : RELATION TO THE MUTATION RESPONSIBLE FOR HIGH EC-SOD LEVEL IN SERUM
- INHIBITION OF HUMAN DIPEPTIDASES BY β-LACTAM ANTIBIOTICS
- EFFECT OF SUPEROXIDE DISMUTASE ON RATS INDUCED KIDNEY INTOXICATION
- DETERMINATION OF ADRIAMYCIN BY ENZYME IMMUNOASSAY
- SUPEROXIDE DISMUTASE LEVELS FOLLOWING LIVER AND KIDNEY INTOXICATION
- SUPEROXIDE DISMUTASE LEVELS FOLLOWING LIVER AND KIDNEY INTOXICATION
- RELATION BETWEEN CARDIOTOXIC EFFECT OF ADRIAMYCIN AND SUPEROXIDE ANION RADICAL
- ENZYME IMMUNOASSAY FOR MANGANESE-SUPEROXIDE DISMUTASE IN SERUM AND URINE
- ENZYME IMMUNOASSAY FOR CUPROZINC-SUPEROXIDE DISMUTASE IN SERUM AND URINE
- RELATION BETWEEN CARDIOTOXIC EFFECT OF ADRIAMYCIN AND SUPEROXIDE DISMUTASE
- PURIFICATION OF SUPEROXIDE DISMUTASES FROM HUMAN PLACENTA USING IMMUNOADSORBENT COLUMNS
- PURIFICATION AND PROPERTIES OF TWO SUPEROXIDE DISMUTASES FROM HUMAN PLACENTA
- NEW METHOD FOR THE ASSAY OF PURINE METABOLIC ENZYMES
- Comparison of the Mechanisms of Cataract Development Involving Differences in Ca^ Regulation in Lenses among Three Hereditary Cataract Model Rats(Biochemistry)
- DIFFERENTIAL DETERMINATION OF CATIONIC AND ANIONIC GLUTATHIONE S-TRANSFERASES BY ENZYME IMMUNOASSAY
- Heparin-Induced Release of Extracellular-Superoxide Dismutase Form (V) to Plasma
- Inhibition of Aminopeptidase N (AP-N) and Urokinase-Type Plasminogen Activator (uPA) by Zinc Suppresses the Invasion Activity in Human Urological Cancer Cells.
- Extract from Serenoa repens Suppresses the Invasion Activity of Human Urological Cancer Cells by Inhibiting Urokinase-Type Plasminogen Activator.
- Inhibition of Aminopeptidase N(AP-N)and Urokinase-Type Plasminogen Activator(uPA)by Zinc Suppresses the Invasion Activity in Human Urological Cancer Cells
- Extract from Serenoa repens Suppresses the Invasion Activity of Human Urological Cancer Cells by Inhibiting Urokinase-Type Plasminogen Activator
- Writing Waveguides in Silica-related Glasses with Femtosecond Laser
- Structure and Antitumor Activity of a Branced (1→3)-β-D-Glucan from the Alkaline Extract of Amanita muscaria.
- Changes in the Heparin Affinity of Extracellular-Superoxide Dismutase in Patients with Coronary Artery Atherosclerosis.
- Changes in the Heparin Affinity of Extracellular-Superoxide Dismutase in Patients with Coronary Artery Atherosclerosis
- An Arginine-213 to Glycine Mutation in Human Extracellular-Superoxide Dismutase Reduces Susceptibility to Trypsin-Like Proteinases
- Toxic Shock Syndrome and Pharmacokinetics of Vancomycin.
- Pharmacokinetics of Granisetron in Adults and Children with Malignant Diseases
- A Rapid Assay of Granisetron in Biological Fluids from Cancer Patients.
- A Rapid Assay of Granisetron in Biological Fluids from Cancer Patients
- The Effect of Ethanolamine on Acute Carbon Tetrachloride Intoxication
- Buformin Suppresses the Expression of Glyceraldehyde 3-Phosphate Dehydrogenase(Biopharmacy)
- Regulators for Blood Glucose Level Affect Gene Expression of Aquaporin 3(Biopharmacy)
- Identification of Differentially Expressed Genes in Hepatic HepG2 Cells Treated with Acetaminophen Using Suppression Subtractive Hybridization(Biochemistry)
- Sentinel node navigation surgery using endoscopic minilaparotomy technique for patients with prostate cancer
- Metabolite Profiles of Flutamide in Serum from Patients with Flutamide-Induced Hepatic Dysfunction(Biopharmacy)
- Myristoleic Acid, a Cytotoxic Component in the Extract from Serenoa repens, Induces Apoptosis and Necrosis in Human Prostatic LNCaP Cells.
- Selection of Mobile Phase in High-Performance Liquid Chromatographic Determination for Medicines.
- Selection of Mobile Phase in High-Performance Liquid Chromatographic Determination for Medicines
- Determination of Human Alkaline Phosphatase Isozymes by Use of Monoclonal Antibodies and their Clinical Application
- AN ENZYMIC MECHANISM FOR MUTAGENIC ACTIVATION OF CARCINOGENIC AMINOAZO DYES
- Tomato Paste Fraction Inhibiting the Formation of Advanced Glycation End-products
- Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantia and Its Degradation Product (TAP-H).
- Antidiabetic Effect of an Acidic Polysaccharide(TAP)from Tremella aurantia and Its Degradation Product(TAP-H)
- Structural Features of an Anti-Diabetic Polysaccharide(TAP) from Tremella aurantia.
- Structural Features of an Anti-Diabetic Polysaccharide (TAP) from Tremella aurantia
- Structural Features and Hypoglycemic Activity of a Polysaccharide(CS-F10) from the Cultured Mycelium of Cordyceps sinensis.
- Activation of Macrophages and Neutrophils by an Endothelium Growth Suppressing Factor.
- Growth Suppressing Factor for Endothelial Cells Exhibits Tumor Regressing Activity.
- Purification of a Growth-Suppressing Factor for Bovine Artery Endothelial Cells from Mouse Lymphoma P388D1 Cells.
- Structural Features and Hypoglycemic Activity of a Polysaccharide (CS-F10) from the Cultured Mycelium of Cordyceps sinensis
- Activation of Macrophages and Neutrophils by an Endothelium Growth Suppressing Factor
- Growth Suppressing Factor for Endothelial Cells Exhibits Tumor Regressing Activity
- Purification of a Growth-Suppressing Factor for Bovine Artery Endothelial Cells from Mouse Lymphoma P388D1 Cells
- Preparation and Antitumor Activities of β-(1→6)Branced(1→3)-β-D-Glucan Derivatives.
- Polysaccharides in Fungi. XXXV. : Anti Diabetic Activity of an Acidic Polysaccharide from the Fruiting Bodies of Tremella aurantia.
- Preparation and Antitumor Activities of Mitomycin C-(1→6)-Branched (1→3)-β-D-Glucan Conjugate.
- Polysaccharides in Fungi. XXXVIII. Anti-diabetic Activity and Structural Feature of a Galactomannan Elaborated by Pestalotiopsis Species
- MOLECULAR ANALYSIS OF EXTRACELLULAR-SUPEROXIDE DISMUTASE GENE ASSOCIATED WITH HIGH LEVEL IN SERUM
- Purification and Characterization of Pranlukast Hydrolase from Rat Liver Microsomes : The Hydrolase is Identical to Carboxylesterase pI 6.2
- Effect of a Polysaccharide(TAP) from the Fruiting Bodies of Tremella aurantia on Glucose Metabolism in Mouse Liver.
- Effect of a Polysaccharide (TAP) from the Fruiting Bodies of Tremella aurantia on Glucose Metabolism in Mouse Liver
- Binding of human xanthine oxidase to sulphated glycosaminoglycans on the endothelial-cell surface.
- Polysaccharides in Fungi. XXXVII. : Immunomodulating Activities of Carboxymethylated Derivatives of Linear (1→3)-α-D-Glucans Extracted from the Fruiting Bodies of Agrocybe cylindracea and Amanita muscaria.
- Polysaccharides in Fungi. XXXVII. Immunomodulating Activities of Carboxymethylated Derivatives of Linear (1→3)-α-D-Glucans Extracted from the Fruiting Bodies of Agrocybe cylindracea and Amanita muscaria
- Polysaccharides in Fungi. XXXIV. : A Polysaccharide from the Fruiting Bodies of Amanita muscaria and the Antitumor Activity of Its Carboxymethylated Product.
- Polysaccharides in Fungi. XXIV. : A(1→3)β-D-Glucan from the Alkaline Extract of the Insect-Body Portion of Chan hua (Fungus : Cordyceps cicadae).
- (1→3)α-D-Glucan from an Alkaline Extract of Agrocybe cylindracea, and Antitumor Activity of its O-(carboxymethyl)ated Derivatives.
- Effect of fundal pressure on maternal to fetal microtransfusion at the time of cesarean delivery
- Solubilization, Purification and Properties of Hexose 6-Phosphate Dehydrogenase from Guinea Pig Liver Microsomes
- DECOMPOSITION OF HYDROPEROXIDES DERIVED FROM MICROSOMES OR LIPOPROTEIN BY GLUTATHIONE PEROXIDASE AND GLUTATHIONE S-TRANSFERASE
- Polysaccharides in Fungi. XXXVIII. : Anti-diabetic Activity and Structural Feature of a Galactomann Elaborated by Pestalotiopsis Species.
- MULTIPLICITY OF CARBONYL REDUCTASE IN GUINEA PIG TISSUES
- EFFECT OF 3-DEOXYGLUCOSONE ON THE ACTIVITIES OF ENZYMES RESPONSIBLE FOR GLUCOSE METABOLISM IN MOUSE LIVER
- Biological Activities of (1→3)-β-D-Glucans with Reducing Glucose Side Chains
- Growth Suppressing Activity for Endothelial Cells Induced from Macrophages by Carboxymethylated Curdlan
- Preparation of Novel (1→3)-β-D-Glucans Having Reducing Glucose Side Chains
- The Recovering Effect of Betaine on Carbon Tetrachloride-Induced Liver Injury
- Preventive Effect of Co-administration of Water Containing Magnesium Ion on Indomethacin Induced Lesions of Gastric Mucosa in Adjuvant-Induced Arthritis Rat