DIFFERENTIAL DETERMINATION OF CATIONIC AND ANIONIC GLUTATHIONE S-TRANSFERASES BY ENZYME IMMUNOASSAY
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概要
- 論文の詳細を見る
Specific enzyme immunoassays for cationic and anionic glutathione S-transferases were established using the specific antibodies which were purified by antigen-bound adsorbent column chromatography. The enzyme immunoassay for cationic glutathione Stransferase had high specificity to cationic enzyme, but showed no cross reactivity with anionic one, and vice versa. The recovery of cationic glutathione S-transferase by the enzyme immunoassay was 94.7%, and coefficient of variation for within day and day-to-day precision were 7.8-10.4% and 8.5-12.5%, respectively. The enzyme immunoassay for anionic glutathione S-transferase also had a good recovery and precision. Using these enzyme immunoassays for glutathione S-transferases, sera of various patients were analyzed. Serum cationic glutathione S-transferase was increased in patients with hepatitis and hepatoma, and anionic glutathione S-transferase in serum was increased in patients with liver cirrhosis.
- 公益社団法人日本薬学会の論文
著者
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HIRANO Kazuyuki
Gifu Pharmaceutical University
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MUTO Yasutoshi
First Department of Internal Medicine, Gifu University School of Medicine
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Adachi T
Laboratory Of Clinical Pharmaceutics Gifu Pharmaceutical University
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Adachi Tetsuo
Gifu Pharmaceutical University
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Muto Yasutoshi
First Department Of Internal Medicine Gifu University School Of Medicine
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SUGIURA MAMORU
Gifu College of Pharmacy
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MIWA TOSHINOBU
Gifu College of Pharmacy
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YAMADA MASAO
First Department of Internal Medicine, Gifu University, School of Medicine
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OKUNO FUMITAKA
First Department of Internal Medicine, Gifu University, School of Medicine
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Adachi Tetsuo
Lab. Of Clinical Pharmaceutics Gifu Pharmaceutical Univ.
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