PURIFICATION OF SUPEROXIDE DISMUTASES FROM HUMAN PLACENTA USING IMMUNOADSORBENT COLUMNS
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概要
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Copper and zinc containing-superoxide dismutase (Cu, Zn-SOD), and manganese containing-superoxide dismutase (Mn-SOD) were purified from human placenta with immunoadsorbent columns using anti-human SOD-antibody conjugated Sepharose 4B. The final preparation has a high purity with a good recovery. The Cu, Zn-SOD purified by an immunoadsorbent column had less zinc content and was more labile than the native Cu, Zn-SOD, but this difference disappeared completely by dialyzing the enzyme obtained by immunoadsorbent column against the buffer containing 1 mM ZnCl_2. Other enzymological properties of two SODs were identical with that of native SODs.
- 社団法人日本薬学会の論文
著者
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HIRANO Kazuyuki
Gifu Pharmaceutical University
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Adachi Tetsuo
Gifu Pharmaceutical University
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ITO YOSHIMASA
Gifu Pharmaceutical University
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SUGIURA MAMORU
Gifu College of Pharmacy
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INOUE HAYAO
Gifu College of Pharmacy
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