Solubilization, Purification and Properties of Hexose 6-Phosphate Dehydrogenase from Guinea Pig Liver Microsomes
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概要
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The solubilization, purification and property of hexose 6-phosphate dehydrogenase from guinea pig liver microsomes is described. The enzyme was effectively solubilized by ammonium hydroxide in the presence of 0.5% detergent, and highly purified. The enzyme showed a different pH dependency for various substrates. The optimum pH for galactose 6-phosphate, glucose 6-phosphate and glucose was 8.5,10.2 and more alkaline, respectively. Km values for the substrates were also changed, pH dependently. The enzyme exhibited the lowest Km values in an order of 10^<-6>M for glucose 6-phosphate at pH 7.0. The enzyme activity was inhibited by CaCl_2 and HgCl_2,but other metal compounds, steroids, pCMB and NADPH did not inhibit the activity. The antibody against the purified enzyme inhibited the enzyme activity of Triton X-100 treated microsomes while the inhibition of the enzyme activity of intact microsomes by antibody was very low.
- 島根大学の論文
著者
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HARA Akira
Department of Pathology, Gifu University School of Medicine
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SAWADA Hideo
Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki Univ
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KATAGIRI Yoshihiro
Department of Pharmacy, Gifu University Hospital
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Sawada Hideo
Department Of Frontier Materials Chemistry Graduate School Of Science And Technology Hirosaki Univer
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HAYASHIBARA MASAKAZU
Department of Pharmacy, Tottori University Hospital
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USUI Shigeyuki
Department of Pharmaceutics, Gifu Pharmaceutical University
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Hara Akira
Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University
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Saeki Takao
Department Of Cardiovascular Disease Research Eisai Tsukuba Research Laboratories
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Saeki Takao
Department Of Pharmacy Shimane Medical University Hospital
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Katagiri Yoshihiro
Department Of Biochemistry Gifu Pharmaceutical University
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Sawada Hideo
Department Of Frontier Materials Chemistry Faculty Of Science And Technology Hirosaki University
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HAYASHIBARA MASAKAZU
Department of Hospital Pharmacy, Tottori University School of Medicine
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SAWADA Hideo
Department of Biochemistry, Gifu College of Pharmacy
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Katagiri Yoshihiro
Deparhnent of Pharmacy Gifu University Hospital
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