The Effect of Membrane Surface Potential on the Permeability of Anionic Compounds Across the Apical Membrane in Human Intestinal Epithelial (Caco-2) Cells
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概要
- 論文の詳細を見る
The effect of membrane surface potential of the apical side on the intracellular uptake of ionic compounds was investigated using the human colon adenocarcinoma cell line (Caco-2). The transepithelial transport of indolepropionic acid and tryptamine was consistent with the uptake behavior shown by rat intestinal brush-border membrane (BBM) vesicles. Imipramine, which diminished the negative charge of the membrane surface (for both Caco-2 and BBM), acted to increase the uptake of the anionic compounds, inodolepropionic acid and ceftibuten, and to decrease that of tryptamine (cationic compound) by both the Caco-2 monolayer and the intestinal BBM vesicles at a pH of 7.5. These results suggest that the effects of membrane surface potential on the permeability of ionic compounds were detectable on the Caco-2 cell line as well as the BBM vesicles. On the other hand, the inhibition of H^+-linked transport and the stimulation of the surface change-regulated uptake of ceftibuten have occurred simultaneously on the Caco-2 cell line in the presence of imipramine. It seems that the membrane surface charge (negative) plays an important role in the transport process of ionic compounds across the intestinal epithelium.
- 公益社団法人日本薬学会の論文
- 1997-07-15
著者
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Sugawara Mitsuru
北海道大学 薬学研究臨床薬剤学
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KOBAYASHI Michiya
Department of Human Health and Medical Sciences, Kochi Medical School
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Kobayashi Michiya
Department Of Human Health And Medical Sciences Kochi Medical School
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Kobayashi Michiya
北海道大学医学部附属病院 薬剤
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Iseki Ken
Department Of Clinical Pharmaceutics And Therapeutics Graduate School-of Pharmaceutical Sciences Hok
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Iseki Ken
Department Of Cell Science Institute Of Biomedical Sciences Fukushima Medical University School Of M
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SUGAWARA Mitsuru
Department of Pharmacy, Hokkaido University Hospital Sapporo
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MIYAZAKI Katsumi
Department of Pharmacy, Hokkaido University Hospital Sapporo
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Sugawara Mitsuru
Department Of Pharmacy Hokkaido University Hospital
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KAIDO Koh-ichi
Department of Pharmacy, Hokkaido University Hospital, School of Medicine, Hokkaido University
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Kaido Koh-ichi
Department Of Pharmacy Hokkaido University Hospital School Of Medicine Hokkaido University
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Miyazaki Katsumi
Department Of Pharmacy Hokkaido University Hospital School Of Medicine Hokkaido University
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Kobayashi Michiya
Department Of Human Health And Medical Science Kochi Medical School
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Miyazaki Katsumi
Department of Biology, Keio University
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