Purification by p-aminobenzoic acid (PABA)-affinity chromatography and the functional reconstitution of the nateglinide/H+ cotransport system in the rat intestinal brush-border membrane
スポンサーリンク
概要
- 論文の詳細を見る
(−)-N-(trans-4-Isopropylcyclohexanecarbonyl)-d-phenylalanine (nateglinide) is a novel oral hypoglycemic agent possessing a peptide-type bond and a carboxyl group in its structure. Recently, we have shown that nateglinide transport occurs via the ceftibuten/H+ cotransport system, which is distinct from PepT1, and that the fluorescein/H+ cotransport system is involved in the uptake of nateglinide. The aim of this study was to characterize the functional properties of the intestinal nateglinide transporter. In the first part of this study, we demonstrated that the ceftibuten/H+ cotransport system is identical to the fluorescein/H+ cotransport system. We succeeded in purification of the nateglinide transporter from brush-border membranes of the rat small intestine using p-aminobenzoic acid (PABA)-affinity chromatography. We then investigated the functional properties of the nateglinide transporter using proteoliposomes prepared from the PABA-affinity chromatography elute. We demonstrated that nateglinide, ceftibuten, and fluorescein are transported by the same transporter in the intestine.
- Elsevier Incの論文
- 2006-02-17
著者
-
Kobayashi Michiya
北海道大学医学部附属病院 薬剤
-
Iseki Ken
北海道大学医学部附属病院 薬剤
-
Itagaki Shirou
Lab. Of Clinical Pharmaceutics & Therapeutics Div. Of Pharmasciences Fac. Of Pharmaceutical Sciences Hokkaido Univ.
-
Kobayashi Michiya
Department Of Pharmacy Hokkaido University Hospital School Of Medicine Hokkaido University
-
Iseki Ken
Department Of Clinical Pharmaceutics & Therapeutics Graduate School Of Pharmaceutical Sciences Hokkaido University
関連論文
- Regulation of Monocarboxylate Transporter 1 in Skeletal Muscle Cells by Intracellular Signaling Pathways
- Pharmacokinetics of Oral and Intravenous Administration of Digoxin after Intestinal Ischemia-Reperfusion
- Liver X receptor regulates expression of MRP2 but not that of MDR1 and BCRP in the liver
- AMP-activated protein kinase regulates the expression of monocarboxylate transporter 4 in skeletal muscle
- Expression and Role of SNAT3 in the Placenta
- Amiodarone Increases the Accumulation of DEA in a Human Alveolar Epithelium-Derived Cell Line(Pharmacology)
- Post-Transcriptional Regulation of Breast Cancer Resistance Protein after Intestinal Ischemia-Reperfusion(Pharmacology)
- A New Method for Determination of Both Thalidomide Enantiomers Using HPLC Systems(Analytical Biochemistry)
- Contribution of Multidrug Resistance-Associated Protein 2 to Secretory Intestinal Transport of Organic Anions(Pharmacology)
- Functional analysis of phenolsulfonphthalein transport system in Long-Evans Cinnamon rats
- The Mechanism of Carrier-Mediated Transport of Folates in BeWo Cells: The Involvement of Heme Carrier Protein 1 in Placental Folate Transport
- Down-Regulation of Intestinal Multidrug Resistance-Associated Protein 2 in Long-Evans Cinnamon Rats
- Long-Evans Cinnamon (LEC) ラットにおける有機アニオンの胆汁排出機構
- 小腸におけるアミオダロン代謝物の排出機構
- Purification by p-aminobenzoic acid (PABA)-affinity chromatography and the functional reconstitution of the nateglinide/H+ cotransport system in the rat intestinal brush-border membrane
- Transepithelial Transport of Telmisartan in Caco-2 Monolayers(Pharmacology)
- Transport Mechanism for L-Lactic Acid in Human Myocytes Using Human Prototypic Embryonal Rhabdomyosarcoma Cell Line (RD Cells)(Pharmacology)
- Characterization of Secretory Intestinal Transport of Phenolsulfonphthalein
- Mechanism of L-lactic Acid Transport in L6 Skeletal Muscle Cells
- Comparison of the Disposition Behavior of Organic anions in an Animal Model for Wilson's Disease (Long-Evans Cinnamon rats) with that in Normal Long-Evans Agouti rats
- Mechanism of Active Secretion of Phenolsulfonphthalein in the Liver via Mrp2 (abcc2), an Organic Anion Transporter
- Single-step isolation method for six glycoforms of human α_1-acid glycoprotein by hydroxylapatite chromatography and study of their binding capacities for disopyramide
- Purification method for α-1-acid glycoprotein with subsequent high-performance liquid chromatographic determination of monosaccharides in plasma of healthy subjects and patients with renal insufficiency
- A Simple Method for the Simulation of Unbound Serum Disopyramide Concentration in Patients
- The Effect of Membrane Surface Potential on the Permeability of Anionic Compounds Across the Apical Membrane in Human Intestinal Epithelial (Caco-2) Cells
- The Transport Mechanism of an Organic Cation, Disopyramide, by Brush-Border Membranes. : Comparison Between Renal Cortex and Small Intestine of the Rat.
- UPTAKE CHARACTERISTICS OF POLYAMINES INTO RAT INTESTINAL BRUSH-BORUER MEMBRANE VESICLES
- MECHANISM OF GASTROINTESTINAL ABSORPTION OF β-LACTAM ANTIBIOTICS : Contribution of Passive Diffusion to the BBM Permeation Process
- Characterization of the Disposition of Lutein after i.v. Administration to Rats(Pharmacology)
- Expression Level of ABCG2 in the Placenta Decreases from the Mid Stage to the End of Gestation
- INTESTINAL ABSORPTION OF SEVERAL β-LACTAM ANTIBIOTICS. V.^ EFFECT OF AMINO β-LACTAM ANALOGUES AND DIPEPTIDES ON THE ABSORPTION OF AMINO β-LACTAM ANTIBIOTICS
- INTESTINAL ABSORPTION MECHANISMS OF SEVERAL ZWITTERIONIC β-LACTAM ANTIBIOTICS : ROLE OF BRUSH BORDER MEMBRANE AND BINDING TO F1 FRACTION
- SEVERAL FINDINGS ON THE MECHANISM OF GASTROINTESTINAL ABSORPTION OF QUATERNARY AMMONIUM COMPOUNDS
- INTERACTION OF TERTIARY AMINES AND QUATERNARY AMMONIUM COMPOUNDS WITH GASTROINTESTINAL MUCIN
- Placental Folate Transport during Pregnancy
- The Mechanism of Carrier-Mediated Transport of Folates in BeWo Cells : The Involvement of Heme Carrier Protein 1 in Placental Folate Transport
- TRANSPORT CHARACTERISTICS OF QUATERNARY AMMONIUM COMPOUNDS ACROSS RAT SMALL INTESTINAL BRUSH BORDER MEMBRANE
- INTESTINAL ABSORPTION OF SEVERAL β-LACTAM ANTIBIOTICS. IV. BINDING TO THE VARIOUS COMPONENTS IN THE INTESTINAL MUCOSA OF RAT AND ROLE IN ABSORPTION PROCESS
- Preparation of Disopyramide Suppository and Its Phamaceutical Evaluation.
- Protective effect of lutein on ischemia-reperfusion injury in rat small intestine.
- Antioxidant Activity of a Novel Extract from Bamboo Grass (AHSS) against Ischemia-Reperfusion Injury in Rat Small Intestine(Pharmacology)
- A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study.
- A full validated hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin in human plasma ultrafiltrates.
- Protective Effect of Soy Isoflavone Genistein on Ischemia-Reperfusion in the Rat Small Intestine