Development and Application of an Enzyme Immunoassay for Karasurin A, an Effective Protein Component of Trichosanthes kirilowii MAX. var. japonicum KITAM.

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概要

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• A method was developed for specific estimation of the content of a non-enzymatic protein, karasurin A, in fractions taken during the extraction and purification processes from a natural source. Anti-karokon serum was elicited in rabbits immunized with fragments of karokon, a dried root tuber of Trichosanthes kirilowii MAX. var. japonicum KITAM. Rabbit antibody specific for karasurin A was identified in anti-karokon serum by the Western blotting method. After separation by SDS-PAGE, protein bands of purified karasurin A and extracted proteins from a medicinal herb which is a karasurin A source were reacted with anti-karokon serum followed by treatment with horseradish peroxidase (HRP)-labeled Fab' of goat anti-rabbit IgG, and then bound HRP-labeled second antibody on protein bands was developed to brown by reaction with a substrate solution of the used enzyme. A novel selected antibody enzyme immunoassay (SAEIA) for karasurin A was developed using selective binding of anti-karasurin A antibody in anti-karokon serum to solid phase karasurin A and HRP-labeled Fab' of the second antibody as the tracer. Specific estimation of the content of karasurin A in several fractions taken during the isolation and purification processes of the protein were possible using the SAEIA method.

• 社団法人日本薬学会の論文
• 1994-03-15

著者

• 荻原 幸夫

  Faculty of Pharmaceutical Sciences, Nagoya City University

• 胡 建国

  Faculty Of Pharmaceutical Sciences Nagasaki University

• 荻原 幸夫

  Faculty Of Pharmaceutical Sciences Nagoya City University

• 北川 常廣

  長崎大学薬学部

• 竹田 忠紘

  Faculty Of Pharmaceutical Sciences Nagoya City University

• 吉田 雅代

  Faculty Of Pharmaceutical Sciences Nagasaki University

• 北川 常廣

  Faculty of Pharmaceutical Sciences, Nagasaki University

• 坂井 温子

  Faculty Of Pharmaceutical Sciences Nagasaki University

• 白 鋼

  Faculty of Pharmaceutical Sciences, Nagasaki University

• 藤原 邦雄

  Faculty of Pharmaceutical Sciences, Nagasaki University

• 藤原 邦雄

  長崎大学薬学部

• 北川 常広

  長崎大学薬学部

• 北川 常廣

  Faculty Of Pharmaceutical Sciences Nagasaki University

• 北川 常広

  長崎大学 薬

• Takeda T

  Kyoritsu University Of Pharmacy

• 北川 常廣

  Faculty Of Pharmaceutical Sciences

• 白 鋼

  長大・薬

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