The Complete Amino Acid Sequence of an Abortifacient Protein, Karasurin

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概要

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• The complete amino acid sequence of a new abortifacient protein, karasurin, was determined. Karasurin, which was isolated from fresh root tubers of Trichosanthes kirilowii MAXIMOWICZ var. japonicum KITAMURA (Cucurbitaceae), was a highly basic protein with pI 10.1 and molecular weight of 28000. Intact karasurin was cleaved with cyanogen bromide, lysyl endopeptidase, formic acid and 2-(2'-nitrophenyl-sulfenyl)-3-methyl-3-bromoindolenine (BNPS-skatole), respectively. Cleavages with N-bromosuccinimide (NBS), trypsin and pepsin were performed for the fragments. The resultant peptide fragments were separated by gel filtration chromatography, reversed-phase high performance liquid chromatography (HPLC) or gel filtration HPLC following sequence analyses by automated Edman methods. Karasurin consists of 246 or 247 amino acid residues with a calculated molecular weight of 27144 or 27215 differing only at the C-terminus with the addition of alanyl residue. Two C-terminal sequences were identified as Asn-Asn-Met-OH and Asn-Asn-Met-Ala-OH by sequence analyses and hydrazinolysis, but there was no micro-heterogeniety in other peptides analysed. The sequence of karasurin revealed a considerable similarity to that of trichosanthin and α-trichosanthin, which are known as abortifacient, ribosome-inactivating and anti human immunodeficiency virus (HIV) (the virus causing aquired immunodeficiency syndrome (AIDS)) proteins, with 93% and 98% identity, respectively.

• 公益社団法人日本薬学会の論文
• 1991-05-25

著者

• 荻原 幸夫

  Faculty of Pharmaceutical Sciences, Nagoya City University

• 若林 克己

  Hormone Assay Center Institute Of Endocrinology Gunma University

• 荻原 幸夫

  Faculty Of Pharmaceutical Sciences Nagoya City University

• 竹田 忠紘

  Faculty Of Pharmaceutical Sciences Nagoya City University

• 加藤 幸雄

  Hormone Assay Center Institute Of Endocrinology Gunma University

• 豊川 峻輔

  Faculty of Pharmaceutical Sciences, Nagoya City University

• 豊川 峻輔

  Faculty Of Pharmaceutical Sciences Nagoya City University

• Takeda T

  Kyoritsu University Of Pharmacy

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