Studies on Viomycin. XV. Comparative Study on the Specificities of Two Anti-viomycin Antisera by Enzyme Immunoassay
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概要
- 論文の詳細を見る
A new viomycin-bovine serum albumin conjugate was characterized, and 19 viomycin molecules were found to be coupled with one molecule of the carrier protein. Enzyme labelling of viomycin with β-D-galactosidase was performed by a continuous two-step process using a maleimide succinimidyl ester type cross-linker. The optical conditions for enzyme immunoassay of viomycin using the new anti-viomycin antiserum and the enzyme labelled viomycin (double antibody method) were studied and a procedure with satisfactory accuracy and precision was developed. The specificities of two anti-viomycin antisera were compared by the enzyme immunoassay technique. Evidence that the enzyme immunoassay procedure is preferable to radioimmunoassay for a comparative study is also presented.
- 公益社団法人日本薬学会の論文
- 1982-07-25
著者
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吉田 憲生
Faculty Of Pharmaceutical Sciences Nagasaki University
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北川 常廣
長崎大学薬学部
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谷森 英明
Faculty of Pharmaceutical Sciences, Nagasaki University
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北川 常廣
Faculty of Pharmaceutical Sciences, Nagasaki University
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藤原 邦雄
Faculty of Pharmaceutical Sciences, Nagasaki University
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浅田 久継
Faculty of Pharmaceutical Sciences, Nagasaki University
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三浦 孝子
Faculty of Pharmaceutical Sciences, Nagasaki University
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藤原 邦雄
長崎大学薬学部
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三浦 孝子
Faculty Of Pharmaceutical Sciences Nagasaki University
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北川 常広
長崎大学薬学部
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北川 常廣
Faculty Of Pharmaceutical Sciences Nagasaki University
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谷森 英明
Faculty Of Pharmaceutical Sciences Nagasaki University
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北川 常広
長崎大学 薬
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北川 常廣
Faculty Of Pharmaceutical Sciences
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浅田 久継
Faculty Of Pharmaceutical Sciences Nagasaki University
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