Contents of Lecithin and Choline in Crude Drugs
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概要
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The determination of lecithin and choline in crude drugs was established by a combination of high performance liquid chromatography (HPLC) with electrochemical detector (ECD) and enzyme reaction. Lecithin in crude drugs extracted with a mixture of chloroform-methanol (2 : 1) at room temperature was hydrolyzed by phospholipase D. The hydrolyzate was injected to HPLC, and choline was separated from impurities by reverse phase column. The choline was converted to betaine and hydrogen peroxide by passing through column packed with immobilized choline oxidase. This hydrogen peroxide was detected by ECD. The peak area of hydrogen peroxide derived from lecithin was proportional to the concentration of lecithin from 0.10 to 1.52 μg/ml. Choline in crude drugs was extracted with ethanol under reflux and determined under the same HPLC conditions as lecithin. The peak area of hydrogen peroxide derived from choline was proportional to the concentration of choline from 0.01 to 0.45 μg/ml. The contents of lecithin and choline in 31 kinds of crude drugs were determined by these established methods. The results showed that Cervi Parvum Cornu, Kokurozin , Foenigraeci Semen and Psoraleae Semen contained more lecithin than other crude drugs, while Angelicae Radix, Foenigraeci Semen, Psoraleae Semen, and especially Hippocampus were found to contain more choline than other crude drugs.
- 社団法人日本薬学会の論文
- 1994-01-15
著者
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西 博行
田辺製薬株式会社分析研究所
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佐藤 忠司
田辺製薬株式会社分析研究所
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宮本 尚明
Analytical Research Laboratory Tanabe Seiyaku Co. Ltd.
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山崎 一矢
Analytical Research Laboratory, Tanabe Seiyaku Co., Ltd.,
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国仙谷 良男
Analytical Research Laboratory, Tanabe Seiyaku Co., Ltd.,
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佐藤 忠司
Analytical Research Laboratory, Tanabe Seiyaku Co., Ltd.,
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松尾 賢明
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd.,
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菊岡 正之
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd.,
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西 博行
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd.,
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松尾 賢明
Analytical Chemistry Research Laboratory Tanabe Seiyaku Co. Ltd.
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西 博行
田辺製薬(株)分析研究所
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西 博行
田辺製薬(株)製品技術研究所分析化学研究部
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山崎 一矢
Analytical Research Laboratory Tanabe Seiyaku Co. Ltd.
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山崎 一矢
田辺製薬株式会社分析化学研究所
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菊岡 正之
Analytical Chemistry Research Laboratory Tanabe Seiyaku Co. Ltd.
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国仙谷 良男
田辺製薬株式会社・製品技術研究所・分析化学研究部
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宮本 尚明
Analytical Chemistry Research Laboratory Tanabe Seiyaku Co. Ltd.
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Maruta Tomoki
Graduate School Of Pharmaceutical Sciences Kyushu University
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