電荷を持つシクロデキストリン誘導体をセレクターとする光学異性体及び疎水性薬物のキャピラリー電気泳動

概要

論文の詳細を見る
Electrokinetic chromatography (EKC), which belongs to one branch of capillary electrophoretic (CE) techniques, enables the separation of electrically neutral compounds by the CE technique through a chromatographic interaction, as in reversed-phase HPLC. In this study we employed several charged cyclodextrin (CD) derivatives as one of the pseudo-stationary phases for EKC. Because these CD derivatives have both chirality and charge, they can be applied to the separation of enantiomers and/or electrically neutral compounds. For more than 40 enantiomers, phosphated (α, β, γ)-CDs, sulfated β-CD, carboxymethylated (β, γ)-CDs, and carboxyethylated (β, γ)-CDs were tested under both acidic and neutral 〜 alkaline conditions in order to investigate the enantioselectivity. Among the tested charged CDs, β-type CDs were effective and about half of the analytes were enantioseparated by EKC with charged CDs, especially with β-CD phosphate or β-CD sulfate. Further, these charged CDs were applied to the separation of some corticosteroids, such as triamsinolone acetonide. These charged CDs were found to be useful as one of the pseudo-stationary phases for EKC.
社団法人日本分析化学会の論文
1998-10-05