Changes in Potassium Permeability and Membrane Potential of Bovine Red Blood Cells estimated by the Use of a Dimerizing Fluorescence Probe

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The partition of 3,3'-dipropyl-2,2'-thiadicarbocyanine, diS-C_3 (5), between the i terior of bovine red cells and bulk aqucous medium was measured by using fluorescence spectroscopy. Inside the cells (concentrated hemoglobin phase), diS-C_3 (5) is intensely concentrated and consequently forms the nonfluorescent dimer. Valinomycin stimulates K^+ transport across the cell membrane and the resulting change in membrane potential induces an alteration of the partitioning and the dimerization of diS-C_3 (5). The changes in membrane potential and permeability of the red cell membrane to K^+ were quantitatively associated with this dimerizatioo. The permeability of the membrane to K^+ estimated here can be correlated with the characteristic properties of bovine red cell membrane, that is, the high content of sphingomyelin and the rigidity of this membrane.
社団法人日本薬学会の論文
1983-04-25