Effects of Oxygen on the Reactivity of Nitrogen Oxide Species Including Peroxynitrite(Biochemistry/Molecular Biology)

概要

論文の詳細を見る
his paper describes the O_2-dependent control of the reactivity of nitrogen oxide species for the production of biologically important nitrated and nitrosated compounds. In this study, the effects of O_2 on the reactivity of NO, NO_2, and ONOO^-/ONOOH for nitration of tyrosine (Tyr) and nitrosation of glutathione (GSH) and morpholine (MOR) were examined. NO produced S-nitrosoglutathione (GSNO) and N-nitrosomorpholine (NMOR) through the formation of N_2O_3 under aerobic conditions, and NO_2 produced 3-nitrotyrosine (3-NO_2Tyr), GSNO, and NMOR. Transnitrosation from GSNO to MOR was observed only in the presence of O_2. Although preformed ONOO^-/ONOOH produced all the products under aerobic conditions, the formation of 3-NO_2Tyr and GSNO was markedly reduced and the formation of NMOR was enhanced under anaerobic conditions. The reactivity of the CO_2 adduct of ONOO^- was similarly dependent on O_2. 3-NO_2Tyr was produced effectively by reaction with ONOO^-/ONOOH at the O_2 concentration of 270μM and by reaction with its CO_2 adduct at O_2 concentrations greater than 5 μM. Generation of・OH from ONOO^-/ONOOH was suppressed under anaerobic conditions. The reactivity of ONOO^-/ONOOH and・OH generation from ONOO^- were reversibly controlled by the O_2 concentration.
公益社団法人日本薬学会の論文
2004-01-01