Examination of the Extraction Methods and Re-evaluation of Blue Fluorescence Generated in Rat Tissues in Situ
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概要
- 論文の詳細を見る
Extraction methods for measuring blue fluorescence generated in rat liver and brain in situ were examined. Great care should be taken when the traditional chloroform/methanol extraction method is employed because artificial blue fluorescence is readily produced by subsequent light irradiation, or by passage through a silicic acid column. In contrast, sodium dodecylsulfate (SDS) extraction were found to be suitable since no artificial blue fluorescence was produced by subsequent light irradiation. The levels of blue fluorescence in rat tissues were re-evaluated following SDS extraction. The levels of blue fluorescence in rat liver increased with age, whereas those in rat brain did not. Accumulation of blue fluorescence in the tissues was only slightly enhanced by vitamin E-deficiency. Blue fluorescence in the extracts did not seem to be derived from autofluorescent yellow lipofuscin in situ. The results were not always consistent with earlier observations obtained using the chloroform/methanol extraction.
- 公益社団法人日本薬学会の論文
- 1994-01-15
著者
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菊川 清見
Tokyo College of Pharmacy
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葛西 洋芳
Tokyo College Of Pharmacy
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加藤 哲太
Tokyo College of Pharmacy
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加藤 哲太
東京薬科大学薬学部
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加藤 哲太
東京薬科大学薬学部薬学科
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菊川 清見
東京薬科大学薬学部
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八巻 真司
Tokyo College of Pharmacy
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