Ganoderma lucidum No.15の産生するアミラーゼの精製とその性質

概要

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A starch-saccharifying enzyme, amylase, extracellularly produced by Ganoderma lucidum No.15 was purified to homogeneity by CM-cellulose chromatography and gel filtration on Bio-gel P-150 and Toyopearl HW 55F, and its properties were examined. The molecular weight of the enzyme was estimated to be 53,000 by gel filtration and 54,000 by SDS-polyacrylamide gel electrophoresis, indicating that it is monomeric. The optimum pH for its activity was 5.0. The optimum temperature was 50℃. The enzyme was stable within the range of 0-40℃ and pH 4.0-9.0. Its isoelectric point was 6.8. The K_m value for soluble starch was 0.83%. The enzyme was capable of hydrolyzing some raw starches but potato. In the case of wheat and corn starches, the starch-digesting rates were about 18.6 and 16.6% of those of cooked starches, respectively. The enzyme was suggested to be glucoamylase because it produced only glucose from starch.
社団法人日本家政学会の論文
1991-06-15