Amycolatopsis sp.CsO-2によるフレーク状キトサン培地において生産されるキトサナーゼ

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An actinomycete, strain CsO-2,capable of producing extracellular chitosanase, was isolated from enrichment cultures in a salt medium containing chitosan flakes. The taxonomic data of strain CsO-2 showed that it belongs to the genus Amycolatopsis. The organism secreted the maximum amount of the enzyme(3μmol glucosamine released/min/ml)when incubated in a medium containing 0.5%(w/v)chitosan flakes and 1.0%(w/v)Polypepton. Crude chitosanase prepared from the culture broth degraded chitosan to glucosamine oligomers, but it could not degrade glucosamine dimer and/or trimer. The extracellular crude chitosanase was stable up to 40℃ after treatment for 10 min, and about 20% of its original activity remained even at 80℃. Cell-free extracts prepared from the organism grown in the chitosan-Polypepton medium degraded chitosan and glucosamine dimer to glucosamine.
社団法人日本生物工学会の論文
1994-01-25