グリセロール培地でアミラーゼを細胞外に多量産生するFusidium sp. BX-1の菌学ならびに生理化学的諸性質

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An imperfect fungus, strain BX-1,capable of utilizing a wide range of carbon sources was isolated from pullulan-enrichment cultures at pH 3.0.Strain BX-1 showed yeast-like growth in liquid media and formed simple or branching chains of elongated blastoconidia on solid media. (It was tentatively designated as Fusidium sp. BX-1.) Strain BX-1 exhibited significant good growth on various saccharides, polyols and oils. When the organism was incubated in a medium containing 2% glycerol as a carbon source, it extraclellularly produced amylases, xylanase, invertase and β-galactosidase. In particular, the produciton of amylases(10〜13 μmol glucose relased/min/ml/ cultuer fluid) was about 3 to 4 times higher than that in the medium containing strach. In addition, the productivry was enhanced by adding a small amount of starch to the glycerol-medium (16〜18 μmol glucose released/min/ml culture fluid), but it was extremely repressed by glucose.
社団法人日本生物工学会の論文
1991-09-25