微小生体試料用低温灰化装置の開発

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Recently in Japan, the low temperature asher is being used popularly for pretreatment of the environmental materials instead of the wet or dry digestion methods. Generally, commercial one is enough large to digest lots of sample. But it is not suitable to small amount of sample such as mycelia. Moreover, it is said that iron, nickel, cromium, etc. are lost during digestion. The authors have developed a new type low temperature asher for small amount of sample and eliminated the loss of metal components. To catch the components which are volatilized from the sample in the boat to the surface of the conventional chamber, an ashing tube has been designed which has both function and as the boat and the chamber in one body. In addition, to make ashing time short, a rotary connector has been induced to take off the ash on the surface of the sample. After digestion in the ashing tube, ash was dissolved with 1 : 1 hydrochloric acid, so the most amount of the metal component could be caught. In consequence of this design, good results were obtained in recovery test as follows; Cd 95%, Pb 98%, Cu 99%, Fe 97%, Ni 98% and Cr 93%. Next it was attempted to apply the apparatus to biological sample, NBS No.1571 orchard leaves. Good results were obtained on manganese, lead, zinc and copper except iron. But the iron's recovery was about 80% against NBS certificated value. It seems that the result is caused by the production of the volatile iron compounds such as pentacarbonyl iron (bp I03℃) and dicarbonyl-dinitrosyl iron (bp 110℃) etc. Thus, it is not unbelievable that the protection of the loss of iron will be performed with increasing the trap capacity of the ashing tube.
社団法人日本分析化学会の論文
1976-04-10

微小生体試料用低温灰化装置の開発

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