炭素炉原子吸光法による生体試料の前処理なし直接定量

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Direct atomization with no pretreatment for the analysis of trace metal elements in intact cell systems and other biological tissues has been tried in carbon rod atomic absorption spectrometry. Silkworm eggs, hatched silkworms, NBS bovine liver, and orchard leaves were selected as illustrative samples. A Hitachi atomic absorption spectrophotometer Model 170-50 with a D_2 lamp background compensation circuit was employed for the measurement. A carbon rod atomizer used was of a Japan-Jarrell-Ash Model FLA-100. Activation analysis was also used for the cross checking of the data. For this, nuclear reactors, TRIGA-MARK II at Rikkyo Univ, and JRR 2-4 at Tokai Atomic Energy Institute, were used along with a pulse height analyzer of Camberra Industries Co. Silkworm eggs and hatched silkworms were mounted onto the atomizer one by one. In the case of bovine liver and orchard leaves, a weighed amount of powder samples were put into the furnace. Calibration curves were made with diluted HNO_3 solution of standard metal ions. A crucial condition for dry (20 A-30 s), ash (70 A-30 s), and atomize (280 A-7 s) cycle was important to get accurate and reproducible absorption peaks. Most of the data obtained by the present method agreed with those by activation analysis for silkworm eggs and hatched silkworms, and with NBS certified or recommended values for orchard leaves and bovine liver. The result suggests that direct atomization with no pretreatment of biological samples is analytically valid in terms of i) high sensitivity, ii) rapidness of measurements, iii) no chance of contamination during the sample pretreatments, and iv) applicability for extremely small amount of samples where pretreatment is virtually impossible. This method is, therefore, particularly effective for discussing the difference in _metal contents in each cell and biological individua.
社団法人日本分析化学会の論文
1977-10-05

炭素炉原子吸光法による生体試料の前処理なし直接定量

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