シロネズミ小腸粘膜におけるビタミンDと結合するたんぱく質について

概要

論文の詳細を見る
For the detection of vitamin D_3-binding protein in rat intestinal mucosa, 2.0ml of mucosal supernatant (105,000×g, 60 min, protein 10mg) was incubated with 15 I.U. (0.97mμmoles, 15×10^4 cpm) of vitamin D_3-1,2-^3H (356mci/mM) in 2℃ for 20 min (total volume 3.0ml, pH 7.0). In the gel filtration of Sephadex G-200 (1.4×55cm), four protein peaks (1〜4) were observed in mucosal cytoplasma, and peak 1 fraction was bound with vitamin D_3-^3H. This peak 1 fraction was disappeared with pretreatment of mucosal supernatant by pronase, and somewhat modified by the RNase and heat treatment (50℃, 5min). DNase did not affect to the vitamin D_3-binding. Peak 1 fraction provided with single band on the polyacrylamide gel disc electrophoresis, and its molecular weight was calculated as about 350,000 (ca. 13S) by the sucrose density gradient centrifugaton. Vitamin D_3-binding protein in an intestinal mucosal cytoplasma was different from that of plasma with behaviours observed by the disc electrophoresis and sucrose density gradient centrifugation. The vitamin D_3 binding was not affected with equal amount of cholesterol, 17β-estradiol and testosterone.
日本ビタミン学会の論文
1970-05-25