ビタミンD依存性カルシウム結合タンパク質の雌成鶏小腸からの二段階精製法

概要

論文の詳細を見る
A new method was developed to isolate quantities of vitamin D-dependent calcium-binding protein (CaBP) from the avian intestine. In contrast to previous method, the necessity of producing rachitic animals was excluded in the present method. The intestinal CaBP level was considerably high in hens (>18 months old) treated with vitamin D_3 and 1,25-dihydroxyvitamin D_3 together. CaBP was purified from the intestinal supernatant of the vitamin D_3/1,25-dihydroxyvitamin D_3-treated hens by a convenient two step procedure. Following partial purification by ammonium sulfate fractionation, CaBP was adsorbed by DEAE-Sephadex A-25 ion exchanger in the presence of EDTA and then selectively eluted from the resin with CaCl_2. This simple procedure provided a 126-fold purification of CaBP over the original supernatant. From the intestine of ten animals 33mg of purified CaBP was obtained with the yield of 42% of the initial amount.
日本ビタミン学会の論文
1986-06-15