1,25-Dihydroxyvitamin D_3リセプターのニワトリ小腸粘膜クロマチンからのマグネシウムイオンによる可溶化

概要

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1,25-Dihydroxyvitamin D_3 receptor was solubilized from hen intestinal chromatin with MgCl_2 contained in a low ionic strength buffer. At low concentrations (≤50mM), MgCl_2 was much more effective in solubilizing the receptor than KCl of an equivalent ionic strength. MgCl_2 was also more effective in this potency than other divalent metal chlorides such as CaCl_2, MnCl_2, and ZnCl_2. The 1,25-dihydroxyvitamin D_3 receptor solubilized with MgCl_2 was similar in its sedimentation property to the receptor solubilized with KCl. In addition to solubilizing the receptor from chromatin, MgCl_2 dissociated the receptor previously bound to DNA at a lower ionic strength than KCl did. These results suggest that magnesium ion interferes with the interaction between the 1,25-dihydroxyvitamin D_3 receptor and its chromatin acceptor site. or DNA via ionic strength-independent manner.
日本ビタミン学会の論文
1986-07-25