カルシウム吸収に関与するカルシウム結合たん白質の種による差異

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Comparative study of vitamin D_3-induced calcium binding protein (BP) was carried out with intestinal mucosa of rat, chick, frog, pig, and carp. The intestinal homogenate in tris buffer, containing 1.37×10^<-2>_M tris-HCl pH7.4,0.119_M NaCl, 4.74×10^<-3>_M KCl and 9.85×10^<-5>_M glucose, was centrifuged 31,000×g for 30 minutes at 0℃. The resulting supernatant was heated at 60℃ for 5 minutes, and then filtered. The filtrate was analyzed for calcium binding activity using ^<45>Ca equilibrated Sephadex G-25 column and subjected to polyacrylamide gel electrophoresis. Calcium binding protein (Ca-BP) was demonstrated in the examined animals. Ca-BP of rat, chick and frog showed the same electrophoretic band which was dependent on vitamin D_3. Ca-BP of pig and carp showed high calcium binding activity compared with the formers, but the electrophoretic band was not observed around the identical region of the formers. Calcium binding protein would be varied with the animal species. (Received June 29,1970)
日本ビタミン学会の論文
1970-09-25