Thiamine Anhydride : (VI)シロネズミにおけるThiamine Anhydrideの代謝産物

概要

論文の詳細を見る
The authors isolated TAO (thiamine anhydride sulfoxide) (I) and 2-methyl-4-amino-5-aminomethylpyrimidine (II) from the urine after intraperitoneal injection of TA (thiamine anhydride) to rats on thiamine-deficient diet. The evaporated urine was extracted by n-butanol, The extract was purified by chromatography using Amberlite IRC-50 and finally from the eluate an alcohol-insoluble crystal of mp. 260℃ and an alcohol-soluble crystal of mp. 130℃ were isolated. The former was identified as II and the latter coincided with I. As both TAO and thiamine anhydride sulfone show the same mp. and Rf on paper chromatography, the metabolite of mp, 130℃ was identified as I by polarography which will be described in the next paper. It is questionable whether II was a real metabolite of TA, because at first no spot coincided with II was not detected by paper chromatography of the original urine and TAO is readily hydrolysed into II by its acid treatment.
日本ビタミン学会の論文
1962-09-25