パパインのビタミンB_1誘導体による阻害 : (II)パパインのThiamine propyldisulfide阻害とパパインSH基との関連

概要

論文の詳細を見る
When papain was incubated with thiamine propyldisulfide (TPD) in increasing concentrations, its enzymatic activity was inhibited proportionally to the thiamine released from TPD. Determination of thiol radicals of TPD-ihibited papain by p-(bis-nitrophenyl) disulfide (PNPD) also indicated decrease of PNPD-values in correspondence with enzymatic activity of the papain. The activity of TPD-inhibited papain, when incubated with ICH_2COOH, was completely destroyed but the thiol radicals detected by PNPD were always demonstrated to the extent of 45-50% of the papain-SH value. When papain was incubated with ICH_2COOH and TPD, thiamine was hardly released and thiol radicals detected by PNPD were also demonstrated to 47% of the papain-SH. When papain was denatured by urea in increasing concentrations, the thiamine released from TPD in its incubation with TPD, was proportional to the enzymatic activity of urea-denatured papain, while the radicals detected by PNPD were always demonstrated. That the thiamine released from TPD in the incubation of papain with TPD was shown to be a good index of the enzymatic activity.
日本ビタミン学会の論文
1968-02-25