ビタミンB_1誘導体の微生物活性 : (III)S-Alkyl-thiamineの安定度ならびに加水分解

概要

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S-Methyl-(I), S-ethyl-(II), S-benzyl-(III), S-3,4,5-trimethoxybenzyl-(IV) and S-triphenyl-methyl-(V) thiamines were shown to be thiochrome negative by the cyanogen bromide-NaOH procedure immediately after preparation of their 10^<-5>M solutions in 1% alcohol. A 10^<-5>M solution of (V) in 1% alcohol was easily hydrolyzed into thiamine at 37℃ to an extent of 28% yield in 24 hours, although the other compounds except (V) were proved to be stable. If (V) is incubated at the concentration of 1/4×10^<-5>M in the broth for L. fermenti or Kl. apiculata without innoculation, it is converted to thiamine to an extent of 57% or 44% yield in 24 hours. The stability of (V) is also influenced by pH, turning more unstable at lower values of pH. Complete conversion of (V) into thiamine, when incubated at 55〜60℃ for 30 minutes, was observed at pH 1 and at the presence of cysteine, the hydrolysis was rather inhibited.
日本ビタミン学会の論文
1963-05-25