Thiothiamineにかんする研究 : (XII)2-Methyl-4-amino-5-aminomethylpyrimidineの肝磨砕液による分解

概要

論文の詳細を見る
2-Methyl-4-amino-5-aminomethylpyrimidine (I), when incubated with a homogenate of rat liver for 3 hours, was degradated, but in an anaerobic condition, (I) was partly recovered unchanged. 2-Methyl-4-amino-5-pyrimidine carboxylic acid (II) from the reaction-mixture was separated by means of paper chromatography and identified as its picrate in comparison with an authentic sample. Another metabolite, presumed to be 2-methyl-4-amino-5-formylpyrimidine (III) was detected on paper chromatogram. Either uptake of oxygen or liberation of ammonia was not clearly demonstrated on incubation of the homogenate with (I) but N_2-saturation or addition of catron, inhibitor of amine-oxidase, markedly inhibited degradation of (I). Enzymatic degradation of (I) to (II) via (III) by rat liver homogenate is partly comparable to monoamine-oxidase but further degradation of (II) or entirely different metabolic pathway of (I) may occur, because (I) was quickly metabolized either by injection of (I) to rats or on incubation of (I) with the liver homogenate.
日本ビタミン学会の論文
1964-05-25