Thiothiamineにかんする研究 : (IX)Thiothiamineの尿中代謝産物よりピリミジン体の分離

概要

論文の詳細を見る
A pyrimidine derivative from the urine was isolated as a metabolite of thiothiamine, when thiothiamine was given parenterally to rats. The urine was extracted with ether and then with butanol, acid and alkaline, respectively. Each extract was concentrated and was extracted with water. The water-soluble fraction separated from an oily residue, showed the presence of four Dragendorff-positive spots on paper chromatography. A pyrimidine derivative was separated as a picrate and after reconversion to hydrochloride of the free base, needles of mp. 260℃ (decomp.) were isolated by using cellulose column chromatography. It was identified as 2-methyl-4-amino-5-aminomethylpyrimidine hydrochloride by mixed melting point, UV spectrum or Rf value and also as picrate (mp. 228℃) which coincided with an authentic sample both in mixed melting point and in elementary analysis.
日本ビタミン学会の論文
1964-05-25