放線菌のキシラナーゼ生産に関する予備的検討 : キシラナーゼの生産に関する研究(第1報)

概要

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Using a strain of Streptomyces sp. No. 3137 which was isolated from soil as a potent producer of xylanase, optimum conditions were investigated for xylanase production.Effect of the composition of medium, cultural conditions, aeration, and agitation on the enzyme production were examined. The volume of aeration and speed of agitation did not severely affect the enzyme production.The Streptomyces sp. produced the xylanase only in the presence of xylan as a carbon source, preferentially in the later non-growing phase, and did not produce it when cultured in the medium contaiing glucose, starch or other carbon sources. Xylanase production seemed to be dependent on the concentration of xylan, increasing linearly with xylan concentration.Enzymatic properties of xylanase were examained using the crude enzyme. The optimum pH of xylanase was 5 to 6 and optimum temperature 50〜55℃. This xylanase was stable over the range of pH 4.0 to 8.0 and also below 55℃ for 30 min.
社団法人日本生物工学会の論文
1976-11-25